Cole E S, Frankel J
Department of Biology, University of Iowa, Iowa City 52242.
Dev Biol. 1991 Dec;148(2):420-8. doi: 10.1016/0012-1606(91)90261-z.
A conjugal block phenotype is described for the Tetrahymena pattern mutant, janA. janA exhibits a characteristic "janus" phenotype in which cells develop with a global mirror-image duplication of the ventral pattern of cortical organelles. janA cells are competent to form mating pairs, but later become irreversibly fused as heteropolar doublets. The few pairs that successfully dissociate fail to undergo postconjugal oral replacement and perish. The janA conjugal block is 100% penetrant, is under prezygotic macronuclear control, and is lethal. Here we characterize this conjugal block genetically and cytologically and demonstrate that it can be rescued by a transferable, wild-type product. New insights into late conjugal events, especially the replacement of the oral apparatus, are reported for wild-type cells as well.
描述了嗜热四膜虫模式突变体janA的接合阻断表型。janA表现出一种特征性的“两面神”表型，即细胞发育时皮层细胞器腹侧模式出现整体镜像重复。janA细胞有能力形成配对，但随后会不可逆地融合成异极双联体。少数成功解离的配对未能进行接合后口器替换并死亡。janA接合阻断的发生率为100%，受接合前大核控制，且具有致死性。在此，我们从遗传学和细胞学角度对这种接合阻断进行了表征，并证明它可被一种可转移的野生型产物拯救。同时也报道了对野生型细胞后期接合事件，尤其是口器替换的新见解。
