Liu Ping-Yen, Chen Jyh-Hong, Lin Li-Jen, Liao James K
Division of Cardiology, Internal Medicine, National Cheng Kung University, Tainan, Taiwan; Cardiovascular Research Center, National Cheng Kung University, Tainan, Taiwan; Vascular Medicine Research Unit, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
Division of Cardiology, Internal Medicine, National Cheng Kung University, Tainan, Taiwan; Cardiovascular Research Center, National Cheng Kung University, Tainan, Taiwan.
J Am Coll Cardiol. 2007 Apr 17;49(15):1619-1624. doi: 10.1016/j.jacc.2006.12.043. Epub 2007 Apr 2.
We sought to determine whether Rho kinase (ROCK) activity is increased in a Taiwanese population with metabolic syndrome (MetS).
Recent studies suggest that ROCK may be involved in the pathogenesis of MetS, but clinical studies linking ROCK with MetS are lacking.
We studied 40 Taiwanese subjects (60% men, mean age 55.5 +/- 5.6 years) who were diagnosed with MetS with National Cholesterol Educational Program Adult Treatment Panel III criteria and 40 age- and gender-matched control subjects. Subject demographics were recorded, and blood samples were obtained.
Compared with control subjects, ROCK activity, as determined by phosphorylation of myosin binding subunit (MBS) in leukocytes, was greater in MetS subjects (mean phospho-MBS/MBS ratio 0.46 vs. 0.35, p = 0.002). A cutoff value for ROCK activity of 0.39 predicted the presence of MetS with specificity and sensitivity rates of 70%. Plasma high-sensitivity C-reactive protein was greater (5.5 mg/l, 95% confidence interval [CI] 3.1 to 7.2 mg/l vs. 2.8 mg/l, 95% CI 1.1 to 3.9 mg/l, p = 0.01) and adiponectin was lower (4.9 microg/ml, 95% CI 3.2 to 6.1 microg/ml vs. 5.9 microg/ml, 95% CI 4.2 to 7.5 microg/ml, p = 0.01) in MetS subjects compared with control subjects, but plasma levels of interleukin-6 and tumor necrosis factor-alpha were not different (p > 0.05 for both). Body mass index, waist circumference, fasting glucose, high-sensitivity C-reactive protein, and triglyceride levels were associated with increased levels of ROCK activity. The risk of increased ROCK activity increased with the number of MetS components (p for trend <0.001).
Rho kinase activity is increased in Taiwanese subjects with MetS and is associated with each component of MetS and markers of inflammation. These findings suggest that ROCK activity may be a novel serological marker of MetS.
我们试图确定在台湾代谢综合征（MetS）人群中Rho激酶（ROCK）活性是否升高。
最近的研究表明ROCK可能参与MetS的发病机制，但缺乏将ROCK与MetS联系起来的临床研究。
我们研究了40名台湾受试者（60%为男性，平均年龄55.5±5.6岁），他们根据美国国家胆固醇教育计划成人治疗小组III标准被诊断为MetS，以及40名年龄和性别匹配的对照受试者。记录受试者的人口统计学数据并采集血样。
与对照受试者相比，通过白细胞中肌球蛋白结合亚基（MBS）的磷酸化测定的ROCK活性在MetS受试者中更高（平均磷酸化MBS/MBS比率为0.46对0.35，p = 0.002）。ROCK活性的临界值为0.39时，预测MetS存在的特异性和敏感性率为70%。与对照受试者相比，MetS受试者的血浆高敏C反应蛋白更高（5.5mg/L，95%置信区间[CI]3.1至7.2mg/L对2.8mg/L，95%CI 1.1至3.9mg/L，p = 0.01），脂联素更低（4.9μg/ml，95%CI 3.2至6.1μg/ml对5.9μg/ml，95%CI 4.2至7.5μg/ml，p = 0.01），但血浆白细胞介素-6和肿瘤坏死因子-α水平无差异（两者p>0.05）。体重指数、腰围、空腹血糖、高敏C反应蛋白和甘油三酯水平与ROCK活性升高相关。ROCK活性增加的风险随MetS组分数量的增加而增加（趋势p<0.001）。
台湾MetS受试者中Rho激酶活性升高，且与MetS的每个组分及炎症标志物相关。这些发现表明ROCK活性可能是MetS的一种新型血清学标志物。
