Salih Barik A, Abasiyanik M Fatih, Ahmed Niyaz
Fatih University, Faculty of Science and Literature, Department of Biology/Microbiology Unit, B.cekmece, Istanbul, Turkey.
Infect Genet Evol. 2007 Jul;7(4):509-12. doi: 10.1016/j.meegid.2007.03.002. Epub 2007 Mar 24.
Helicobacter pylori genetic diversity affects the function and antigenicity of virulence factors associated with the disease outcome. Gene profile was done to identify the distribution of gene loci within and outside the cag pathogenicity-island (PAI). H. pylori strains from 35 patients [21 gastritis, 14 peptic ulcer diseases (PUD)] were analyzed using PCR. The profile of the cag PAI was evaluated using primers spanning the 3' end, cagA, promoter region of the cagA, cagE, cagT, 5' end (LEC), extreme right end, plasticity region open reading frames (ORFs), oipA (Hp0638) and vacA alleles. We found few intact cag PAI in the strains examined. Deletions were found in LEC1 (9.5% versus 14.3%), LEC2 (4.8% versus 14.3%), cagT (33.3% versus 28.6%), cagE (28.6% versus 28.6%) and the promoter region of the cagA (19.0% versus 42.9%) of gastritis and PUD strains, respectively. The cagA gene was detectable in 57.1% of gastritis and 92.9% of PUD-associated strains. The cagRJ region also showed deletions for many of its genes. The oipA (Hp0638) gene was detected in 80.9% of gastritis and in 92.9% of PUD strains. The plasticity region ORFs JHP912 and JHP931 were predominant in PUD strains. The vacA-s1a-m1a genotype was predominant in PUD, while s2m2 in gastritis strains. This comprehensive analysis showed deletions in several genes within and outside the cag PAI. However, cagA, oipA, JHP912, JHP931 and vacA-s1a-m1a were more predominant in PUD strains than gastritis-associated strains, suggesting the importance of genetic diversity on the disease progression and clinical outcome.
幽门螺杆菌的基因多样性会影响与疾病转归相关的毒力因子的功能和抗原性。通过基因分型来确定cag致病岛（PAI）内外基因座的分布情况。使用聚合酶链反应（PCR）对来自35例患者[21例胃炎、14例消化性溃疡疾病（PUD）]的幽门螺杆菌菌株进行分析。使用跨越cag PAI 3'端、cagA、cagA启动子区域、cagE、cagT、5'端（LEC）、最右端、可塑性区域开放阅读框（ORF）、oipA（Hp0638）和vacA等位基因的引物来评估cag PAI的基因图谱。我们在所检测的菌株中发现很少有完整的cag PAI。在胃炎和PUD菌株中，分别在LEC1（9.5%对14.3%）、LEC2（4.8%对14.3%）、cagT（33.3%对28.6%）、cagE（28.6%对28.6%）以及cagA启动子区域（19.0%对42.9%）发现了缺失。在57.1%的胃炎菌株和92.9%的与PUD相关的菌株中可检测到cagA基因。cagRJ区域的许多基因也显示出缺失。在80.9%的胃炎菌株和92.9%的PUD菌株中检测到oipA（Hp0638）基因。可塑性区域ORF JHP912和JHP931在PUD菌株中占主导地位。vacA - s1a - m1a基因型在PUD中占主导地位，而s2m2在胃炎菌株中占主导地位。这项综合分析显示cag PAI内外的几个基因存在缺失。然而，cagA、oipA、JHP912、JHP931和vacA - s1a - m1a在PUD菌株中比在与胃炎相关的菌株中更占优势，这表明基因多样性对疾病进展和临床转归具有重要意义。
