Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas, USA.
J Clin Microbiol. 2012 Feb;50(2):441-8. doi: 10.1128/JCM.00906-11. Epub 2011 Nov 23.
The plasticity region of Helicobacter pylori is a large chromosomal segment including isolate-specific open reading frames with characteristics of pathogenicity islands. It remains unclear whether genes in the plasticity region play a role in the pathogenesis of gastric mucosal inflammation and gastroduodenal disease. Our aim was to assess the role of selected genes in the plasticity region in relation to risk of H. pylori-related disease and the severity of gastric mucosal damage. We used PCR to study the relation of disease outcome and mucosal damage with four genes in the H. pylori plasticity region (jhp0940, jhp0945, jhp0947, and jhp0949) from isolates obtained from both Western (n = 296) and East Asian (n = 217) patients. The prevalence of jhp0945, jhp0947, and jhp0949 differed significantly between Western and East Asian isolates. In Western isolates, the presence of jhp0945 was significantly associated with gastric ulcer, duodenal ulcer, and gastric cancer (odds ratios [95% confidence intervals]: 2.27 [1.04 to 4.98], 1.86 [1.03 to 3.34], and 1.92 [1.03 to 3.56], respectively). jhp0940-positive Western isolates were significantly associated with absence of gastric ulcer or duodenal ulcer (0.21 [0.05 to 0.94] and 0.31 [0.12 to 0.78], respectively). No significant difference was observed between inflammatory cell infiltration or atrophy and the presence or absence of plasticity region genes. The outcome of H. pylori infections varies widely geographically. These data suggest a possible role for difference in the prevalence of plasticity region genes in the geographic variation in H. pylori-related diseases.
幽门螺杆菌的可塑性区域是一个包括分离株特异性开放阅读框的大染色体片段，具有致病岛的特征。目前尚不清楚可塑性区域中的基因是否在胃黏膜炎症和胃肠疾病的发病机制中起作用。我们的目的是评估可塑性区域中的选定基因在与 H. pylori 相关疾病的风险和胃黏膜损伤的严重程度之间的作用。我们使用 PCR 研究了来自西方（n = 296）和东亚（n = 217）患者分离株的 H. pylori 可塑性区域（jhp0940、jhp0945、jhp0947 和 jhp0949）中的四个基因与疾病结局和黏膜损伤的关系。jhp0945、jhp0947 和 jhp0949 在西方和东亚分离株中的流行率存在显著差异。在西方分离株中，jhp0945 的存在与胃溃疡、十二指肠溃疡和胃癌显著相关（比值比[95%置信区间]：2.27[1.04 至 4.98]、1.86[1.03 至 3.34]和 1.92[1.03 至 3.56]）。jhp0940 阳性的西方分离株与无胃溃疡或十二指肠溃疡显著相关（0.21[0.05 至 0.94]和 0.31[0.12 至 0.78]）。可塑性区域基因的存在或不存在与炎症细胞浸润或萎缩之间未观察到显著差异。H. pylori 感染的结局在地理上差异很大。这些数据表明，可塑性区域基因的流行率差异可能在 H. pylori 相关疾病的地理变异中起作用。
