Cansizoglu Ahmet E, Lee Brittany J, Zhang Zi Chao, Fontoura Beatriz M A, Chook Yuh Min
Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park, Dallas, Texas 75390-9041, USA.
Nat Struct Mol Biol. 2007 May;14(5):452-4. doi: 10.1038/nsmb1229. Epub 2007 Apr 15.
Kapbeta2 (also called transportin) recognizes PY nuclear localization signal (NLS), a new class of NLS with a R/H/Kx((2-5))PY motif. Here we show that Kapbeta2 complexes containing hydrophobic and basic PY-NLSs, as classified by the composition of an additional N-terminal motif, converge in structure only at consensus motifs, which explains ligand diversity. On the basis of these data and complementary biochemical analyses, we designed a Kapbeta2-specific nuclear import inhibitor, M9M.
Kapβ2（也称为转运蛋白）识别PY核定位信号（NLS），这是一类新的具有R/H/Kx((2-5))PY基序的NLS。我们在此表明，根据额外N端基序的组成分类，含有疏水和碱性PY-NLS的Kapβ2复合物仅在共有基序处结构趋同，这解释了配体多样性。基于这些数据和补充生化分析，我们设计了一种Kapβ2特异性核输入抑制剂M9M。
