Parthimos T, Tsopanakis C, Angelogianni P, Schulpis K H, Parthimos N, Tsakiris S
Department of Experimental Physiology, Medical School, University of Athens, Athens, Greece.
Int J Sports Med. 2007 Aug;28(8):650-4. doi: 10.1055/s-2007-964855. Epub 2007 Apr 13.
The aim of this study was to investigate whether the activities of erythrocyte membrane acetylcholinesterase (AChE), (Na (+),K (+))-ATPase and Mg (2+)-ATPase are modulated by a basketball training. Blood was obtained from 10 basketball players pre- and postexercise. Total antioxidant status (TAS), lactate and pyruvate concentrations were determined with kits, while the enzyme activities were determined spectrophotometrically. Post-training blood lactate and pyruvate concentrations as well as AChE (2.90 +/- 0.05 vs. 3.98 +/- 0.09 Delta OD/min . mg protein, p < 0.01) and Na (+),K (+)-ATPase (0.58 +/- 0.04 vs. 1.27 +/- 0.12 micromol Pi/h . mg protein, p < 0.001) activities were remarkably increased, whereas TAS was significantly decreased. Mg (2+)-ATPase activity remained unaltered at the end of the training. In conclusion, the stimulation of AChE and Na (+),K (+)-ATPase by the training may be due to the rise of blood catecholamine oxidation contributing to TAS decrease and/or the increase of serotonin levels. This stress condition may modulate cholinergic and catecholaminergic/serotoninergic functions in players.
本研究旨在调查篮球训练是否会调节红细胞膜乙酰胆碱酯酶（AChE）、（Na⁺，K⁺）-ATP酶和Mg²⁺-ATP酶的活性。在运动前后从10名篮球运动员身上采集血液。使用试剂盒测定总抗氧化状态（TAS）、乳酸和丙酮酸浓度，同时用分光光度法测定酶活性。训练后血液中的乳酸和丙酮酸浓度以及AChE（2.90±0.05对3.98±0.09ΔOD/min·mg蛋白质，p<0.01）和（Na⁺，K⁺）-ATP酶（0.58±0.04对1.27±0.12μmol Pi/h·mg蛋白质，p<0.001）的活性显著增加，而TAS显著降低。训练结束时Mg²⁺-ATP酶活性保持不变。总之，训练对AChE和（Na⁺，K⁺）-ATP酶的刺激可能是由于血液儿茶酚胺氧化增加导致TAS降低和/或血清素水平升高。这种应激状态可能会调节运动员的胆碱能和儿茶酚胺能/血清素能功能。
