Department of Physiology, Medical School, Athens University, Greece.
Scand J Clin Lab Invest. 2010 Dec;70(8):568-74. doi: 10.3109/00365513.2010.527365. Epub 2010 Oct 22.
To investigate erythrocyte membrane AChE, Na(+), K(+)-ATPase and Mg(2+)-ATPase activities in mothers and their full-term or premature newborns in relation to the mode of delivery.
Blood was obtained from mothers pre- and post-delivery and the umbilical cord (CB) of their full-term newborns: Group A₁ (n = 16) born with vaginal delivery (VD), Group B₁ (n = 14) full-terms with scheduled cesarean section (CS), Group A₂ (n = 12) prematures with VD, Group B₂ (n = 14) prematures with CS. Total Antioxidant Status (TAS) and common laboratory tests were measured with routine methods, and the membrane enzyme activities spectrophotometrically.
TAS was reduced in mothers post VD and in the CB whereas remained unaltered in CS mothers and their newborns. AChE and Na(+), K(+)-ATPase were increased in mothers post VD. AChE was lower in the CB of prematures than that of full-terms independently of the mode of delivery. Na(+), K(+)-ATPase activity was increased in the groups of mothers post VD and decreased in prematures. The enzyme was higher in prematures with CS than that with VD. Mg(2+)-ATPase activity was unchanged.
The increased maternal AChE and Na(+), K(+)-ATPase activities may be due to the low TAS determined post VD, whereas their decreased activities in prematures to their immaturity.
研究红细胞膜乙酰胆碱酯酶（AChE）、Na(+)、K(+)-ATP 酶和 Mg(2+)-ATP 酶在与分娩方式有关的母亲及其足月或早产儿中的活性。
在母亲分娩前和分娩后以及足月新生儿的脐血（CB）中采集血液：A₁ 组（n = 16）经阴道分娩（VD），B₁ 组（n = 14）足月择期剖宫产（CS），A₂ 组（n = 12）早产儿经 VD 分娩，B₂ 组（n = 14）早产儿行 CS。用常规方法测量总抗氧化状态（TAS）和常见实验室检查，并用分光光度法测量膜酶活性。
VD 分娩后母亲和 CB 中的 TAS 降低，而 CS 母亲及其新生儿的 TAS 保持不变。VD 分娩后母亲的 AChE 和 Na(+)、K(+)-ATP 酶增加。无论分娩方式如何，早产儿的 CB 中的 AChE 均低于足月儿。VD 分娩后，母亲组的 Na(+)、K(+)-ATP 酶活性增加，早产儿的 Na(+)、K(+)-ATP 酶活性降低。CS 分娩的早产儿的酶活性高于 VD 分娩的早产儿。Mg(2+)-ATP 酶活性保持不变。
VD 分娩后母亲的 AChE 和 Na(+)、K(+)-ATP 酶活性增加可能是由于 TAS 降低所致，而早产儿的酶活性降低可能与其不成熟有关。
