Hoffman-Goetz L, Spagnuolo P A
Department of Health Studies and Gerontology, University of Waterloo, Waterloo, Ontario, Canada.
Int J Sports Med. 2007 Sep;28(9):787-91. doi: 10.1055/s-2007-964857. Epub 2007 Apr 13.
The purpose of this study was to examine the effect of voluntary training in mice on the expression of apoptotic proteins in splenic lymphocytes following acute exercise. Thirty-three mice were randomized to four treatments: 1) control group (no training and no acute exercise), 2) training, no acute exercise, 3) no training, acute exercise and 4) training, acute exercise. Mice were sacrificed immediately after acute exercise. Western immunoblotting was used to detect apoptosis in splenic lymphocytes for concentrations of anti- (Bcl-2) and pro-apoptotic (caspase 3) proteins. Plasma corticosterone was used as an index of exercise stress. Trained mice not given acute exercise challenge had elevated Bcl-2 (p < 0.05) and lower caspase 3 (p < 0.05) levels relative to control mice. Following the acute exercise challenge, however, trained and untrained mice did not differ in the concentrations of these proteins in splenocytes. Thus, freewheel exercise training in mice reduces splenic lymphocyte apoptosis when tissue samples are obtained at rest but not after acute exercise.
本研究的目的是检测小鼠自愿训练对急性运动后脾淋巴细胞凋亡蛋白表达的影响。33只小鼠被随机分为四组进行处理：1）对照组（无训练且无急性运动），2）训练组，无急性运动，3）无训练，急性运动，4）训练，急性运动。急性运动后立即处死小鼠。采用蛋白质免疫印迹法检测脾淋巴细胞中抗凋亡蛋白（Bcl-2）和促凋亡蛋白（caspase 3）的浓度，以检测细胞凋亡情况。血浆皮质酮用作运动应激指标。与对照组小鼠相比，未接受急性运动挑战的训练小鼠Bcl-2水平升高（p<0.05），caspase 3水平降低（p<0.05）。然而，在急性运动挑战后，训练组和未训练组小鼠脾细胞中这些蛋白质的浓度没有差异。因此，当在休息时获取组织样本时，小鼠的自由运动训练可减少脾淋巴细胞凋亡，但在急性运动后则不然。
