School of Public Health and Health Systems, University of Waterloo, Waterloo, ON, Canada.
Can J Aging. 2012 Jun;31(2):161-71. doi: 10.1017/S0714980812000104.
Aging is associated with increased intestinal inflammation and elevated risk of chronic diseases including inflammatory bowel diseases and colon cancer; many epidemiologic studies show that regular exercise reduces risk. This study examined the effects of long-term voluntary exercise on inflammatory mediators expressed in the intestine of older (15-16 months), healthy C57BL/6 mice. Animals were assigned to four months of freewheel running (WR; n = 20) or to a "sedentary" no wheel running (NWR; n = 20) control group. Intestinal lymphocytes were harvested and analysed for expression of (1) pro-inflammatory (TNF-α, IL-1β) and pleiotropic (IL-6) cytokines, and (2) pro-(caspase-3/-7) and anti-(Bcl-2) apoptotic proteins. Training was confirmed by skeletal muscle enzyme activity; stress was assessed by plasma 8-iso-PGF(2α) and corticosterone. The WR mice had a lower expression of TNF-α, caspase-7, and 8-isoprostanes (p < .05) compared to sedentary controls, suggesting that long-term exercise may "protect" the bowel by reducing inflammatory cytokine and apoptotic protein expression.
衰老是与肠道炎症增加和慢性疾病风险升高相关的，包括炎症性肠病和结肠癌；许多流行病学研究表明，有规律的运动可以降低风险。本研究检测了长期的自愿运动对 15-16 月龄健康 C57BL/6 老年小鼠肠道中表达的炎症介质的影响。动物被分配到四个月的自由轮跑（WR；n = 20）或“久坐”无轮跑（NWR；n = 20）对照组。采集肠道淋巴细胞并分析（1）促炎（TNF-α，IL-1β）和多效性（IL-6）细胞因子，以及（2）促凋亡（caspase-3/-7）和抗凋亡（Bcl-2）蛋白的表达。训练通过骨骼肌酶活性来证实；通过血浆 8-异前列腺素 F2α（8-iso-PGF(2α)）和皮质酮评估应激。与久坐对照组相比，WR 组小鼠 TNF-α、caspase-7 和 8-异前列腺素的表达较低（p <.05），这表明长期运动可能通过降低炎症细胞因子和凋亡蛋白的表达来“保护”肠道。
