School of Public Health and Health Systems, Faculty of Applied Health Sciences, University of Waterloo, Ontario, Canada.
Exerc Immunol Rev. 2012;18:142-57.
Exhaustive exercise induces apoptosis and oxidative stress in systemic organs and tissues and is associated with increased levels of pro-inflammatory cytokines. The effects of acute exercise on cytokine expression and apoptosis of immune cells in the central nervous system (CNS) have not been well characterized.
We investigated the effects of a single bout of strenuous exercise on the expression of TNF-alpha, IL-6, and IL-beta, as well as the apoptotic status of cells in the hippocampus of healthy mice. To compare central vs. systemic differences, cytokine expression in the intestinal lymphocytes of a subset of mice were also assessed.
Female C57BL/6 mice were divided into three groups: sedentary controls (NOTREAD) (n = 22), treadmill exercise with immediate sacrifice (TREAD-Imm) (n = 21), or treadmill exercise with sacrifice after 2 hours (TREAD-2h). TNF-alpha, IL-6, and IL-1beta expression in the hippocampus and intestinal lymphocytes were measured by Western blot analysis. Percentages of hippocampal cells undergoing apoptosis (Annexin+) or necrosis (Propidium Iodide+) were determined through flow cytometry. Plasma levels of 8-isoprostane and corticosterone were measured using commercially available EIA kits.
Acute treadmill exercise led to significant decreases in TNF-alpha (p<0.05) and increases in IL-6 (p<0.05) expression in the hippocampus of healthy mice. No effects of acute exercise on the apoptotic status of hippocampal cells were observed. In intestinal lymphocytes, the exercise bout led to significant increases in TNF-alpha (p<0.05), IL-6 (p<0.05), and IL-1beta (p<0.05). Acute exercise was associated with a significant increase in both plasma 8-isoprostane (p<0.05) and corticosterone (p<0.05) levels.
Acute exercise differentially affects the pattern ofpro-inflammatory cytokine expression in the hippocampus compared to intestinal lymphocytes and, further, does not induce apoptosis in hippocampal cells.
剧烈运动可导致全身器官和组织中的细胞凋亡和氧化应激，并与促炎细胞因子水平升高有关。急性运动对中枢神经系统（CNS）中免疫细胞的细胞因子表达和凋亡的影响尚未得到很好的描述。
我们研究了剧烈运动对健康小鼠海马中 TNF-α、IL-6 和 IL-β表达以及细胞凋亡状态的影响。为了比较中枢与全身的差异，还评估了一部分小鼠肠淋巴细胞中的细胞因子表达。
将雌性 C57BL/6 小鼠分为三组：安静对照组（NOTREAD）（n = 22）、跑步机运动即刻处死组（TREAD-Imm）（n = 21）或跑步机运动 2 小时后处死组（TREAD-2h）。通过 Western blot 分析测量海马和肠淋巴细胞中 TNF-α、IL-6 和 IL-1β的表达。通过流式细胞术测定海马细胞凋亡（Annexin+）或坏死（碘化丙啶+）的百分比。使用市售的 EIA 试剂盒测量血浆中 8-异前列腺素和皮质酮的水平。
急性跑步机运动导致健康小鼠海马中 TNF-α（p<0.05）显著降低，IL-6（p<0.05）表达增加。急性运动对海马细胞的凋亡状态没有影响。在肠淋巴细胞中，运动使 TNF-α（p<0.05）、IL-6（p<0.05）和 IL-1β（p<0.05）表达显著增加。急性运动与血浆 8-异前列腺素（p<0.05）和皮质酮（p<0.05）水平的显著升高有关。
急性运动对海马中促炎细胞因子表达模式的影响与肠淋巴细胞不同，并且不会诱导海马细胞凋亡。
