Parth K, Zimprich H, Brunel R
Acta Endocrinol (Copenh). 1976 Feb;81(2):330-9. doi: 10.1530/acta.0.0810330.
An accurate and relatively simple radioimmunoassay for the determination of aldosterone concentration in peripheral plasma has been developed, 0.5-2.0 ml plasma with added [1,2-3H]aldosterone is extracted with dichloromethane. Purification of the extract is achieved by thin later chromatography in the system benzene-acetone 1:1. Recovery of [1,2-3H] aldosterone is 58 +/- 6 (SD)%. Bound and free fractions are separated by dextran-coated charcoal. The intra-assay reproducibility is 8.8% and the inter-assay reproducibility varies from 11.4-16.1%. The sensitivity of the assay for a 5 ml plasma sample can be put at 0.2 ng/100 ml. Normal values determined in 52 healthy children of different age groups are presented. Furthermore the aldosterone stimulating effect of low sodium diet (17 children), severe and prolonged vomiting (19 children) and synthetic ACTH (10 children) has been studied by our modified method.
已开发出一种准确且相对简单的放射免疫分析法，用于测定外周血浆中的醛固酮浓度。向0.5 - 2.0毫升血浆中加入[1,2 - 3H]醛固酮后，用二氯甲烷萃取。萃取物通过在苯 - 丙酮1:1系统中的薄层层析进行纯化。[1,2 - 3H]醛固酮的回收率为58±6（标准差）%。结合和游离部分通过葡聚糖包被活性炭分离。批内重复性为8.8%，批间重复性在11.4 - 16.1%之间变化。该分析法对5毫升血浆样本的灵敏度可达0.2纳克/100毫升。给出了在52名不同年龄组健康儿童中测定的正常值。此外，还通过我们改进的方法研究了低钠饮食（17名儿童）、严重且长期呕吐（19名儿童）和合成促肾上腺皮质激素（10名儿童）对醛固酮的刺激作用。
