Drewa Tomasz, Krawczyk Agnieszka, Gospodarek Eugenia, Jachna-Sawicka Katarzyna, Bugalski Roman, Kierzenkowska-Mila Celestyna
Department of Tissue Engineering, Chair of Medical Biology, N. Copernicus University, Bydgoszcz, Poland.
Acta Pol Pharm. 2006 Nov-Dec;63(6):543-6.
Staphylococcin T (StT) is a bacteriocin, which can serve as an antibiotic. The influence of StT on the mammalian cells viability should be examined before its possible applications. The aim of this work was to study the influence of StT on mouse fibroblasts viability in vitro. StT was delivered as a deposit in agar. 3T3 fibroblasts were used in viability tests. Cells were incubated with StT for 24 and 48 h. In the first controls, cells grew without the agar block, in the second controls, the agar block contained no StT. Trypan blue exclusion test was performed. Growth inhibition of sensitive strain SAU-209P was observed after 24 h incubation with cell culture medium containing StT. Antimicrobial activity of StT in culture medium was confirmed even after 2 months. The viability of 3T3 cells in the second control group was 50% lower than in the first and 25% higher than in StT treated group during the first day. It was noticed mechanical damage of growing cells caused by agar blocks. The number of cells cultured with StT was similar to that of second control group after 2 days. There were no changes in morphology of cells in all groups. It follows that StT had unimportant influence on the fibroblasts (3T3) viability in culture. StT may be considered for future animal trails.
葡萄球菌素T（StT）是一种细菌素，可作为抗生素使用。在其可能的应用之前，应检测StT对哺乳动物细胞活力的影响。这项工作的目的是研究StT对体外培养的小鼠成纤维细胞活力的影响。StT以琼脂中的沉淀物形式提供。在活力测试中使用了3T3成纤维细胞。将细胞与StT孵育24小时和48小时。在第一个对照组中，细胞在没有琼脂块的情况下生长，在第二个对照组中，琼脂块不含StT。进行了台盼蓝排斥试验。用含有StT的细胞培养基孵育24小时后，观察到敏感菌株SAU - 209P的生长受到抑制。即使在2个月后，也证实了StT在培养基中的抗菌活性。在第一天，第二个对照组中3T3细胞的活力比第一个对照组低50%，比StT处理组高25%。注意到琼脂块对生长中的细胞造成了机械损伤。2天后，用StT培养的细胞数量与第二个对照组相似。所有组细胞的形态均无变化。由此可见，StT对培养中的成纤维细胞（3T3）活力影响不大。未来可考虑对StT进行动物试验。
