Oron Amir, Oron Uri, Streeter Jackson, de Taboada Luis, Alexandrovich Alexander, Trembovler Victoria, Shohami Esther
Department of Orthopedics, Assaf Harofeh Medical Center, Zerifin, Israel.
J Neurotrauma. 2007 Apr;24(4):651-6. doi: 10.1089/neu.2006.0198.
Low-level laser therapy (LLLT) has been evaluated in this study as a potential therapy for traumatic brain injury (TBI). LLLT has been found to modulate various biological processes. Following TBI in mice, we assessed the hypothesis that LLLT might have a beneficial effect on their neurobehavioral and histological outcome. TBI was induced by a weight-drop device, and motor function was assessed 1 h post-trauma using a neurological severity score (NSS). Mice were then divided into three groups of eight mice each: one control group that received a sham LLLT procedure and was not irradiated; and two groups that received LLLT at two different doses (10 and 20 mW/cm(2) ) transcranially. An 808-nm Ga-As diode laser was employed transcranially 4 h post-trauma to illuminate the entire cortex of the brain. Motor function was assessed up to 4 weeks, and lesion volume was measured. There were no significant changes in NSS at 24 and 48 h between the laser-treated and non-treated mice. Yet, from 5 days and up to 28 days, the NSS of the laser-treated mice were significantly lower (p < 0.05) than the traumatized control mice that were not treated with the laser. The lesion volume of the laser treated mice was significantly lower (1.4%) than the non-treated group (12.1%). Our data suggest that a non-invasive transcranial application of LLLT given 4 h following TBI provides a significant long-term functional neurological benefit. Further confirmatory trials are warranted.
在本研究中，低强度激光疗法（LLLT）已被评估为创伤性脑损伤（TBI）的一种潜在治疗方法。已发现LLLT可调节各种生物学过程。在小鼠发生TBI后，我们评估了LLLT可能对其神经行为和组织学结果产生有益影响的假设。通过重物坠落装置诱导TBI，并在创伤后1小时使用神经严重程度评分（NSS）评估运动功能。然后将小鼠分成每组八只的三组：一组为接受假LLLT程序且未接受照射的对照组；另外两组在创伤后4小时经颅接受两种不同剂量（10和20 mW/cm²）的LLLT。使用808纳米的砷化镓二极管激光在创伤后4小时经颅照射大脑的整个皮质。评估长达4周的运动功能，并测量损伤体积。在激光治疗组和未治疗组之间，24小时和48小时时NSS没有显著变化。然而，从第5天到第28天，激光治疗小鼠的NSS显著低于未接受激光治疗的创伤对照组小鼠（p<0.05）。激光治疗小鼠的损伤体积显著低于未治疗组（1.4%对12.1%）。我们的数据表明，在TBI后4小时进行非侵入性经颅应用LLLT可提供显著的长期功能性神经益处。有必要进行进一步的验证试验。
