Yoshino Takashi, Laine Marja L, van Winkelhoff Arie Jan, Dahlén Gunnar
Department of Oral Microbioloy, Institute of Odontology, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
FEMS Microbiol Lett. 2007 May;270(1):75-81. doi: 10.1111/j.1574-6968.2007.00651.x.
Porphyromonas gingivalis is considered an important pathogen in periodontal disease. While this organism expresses a number of virulence factors, no study combining different virulence polymorphisms has, so far, been conducted. The occurrence of combined virulence (Cv) genotypes in 62 isolates of P. gingivalis was investigated from subjects displaying either chronic periodontitis or periodontal abscess. The Cv genotypes, based on gene variation of fimbriae (fimA), Lys-specific cystein proteinase (kgp) and Arg-specific cystein proteinase (prpR1/rgpA), were evaluated by PCR. The isolates were also subjected to capsular polysaccharide K-serotyping. A total of 18 Cv genotype variants based on fimA: kgp: rgpA were identified, of which II:I:A and II:II:A Cv genotypes (53.3%) were the two most frequently detected combinations. Moreover, 36% of the isolates were K-typeable, with the K6 serotype being the most prevalent (23%). Two isolates had the same genotype as the virulent strain W83. The results indicate that chronic periodontitis is not associated with a particularly virulent clonal type. A highly virulent genotype (e.g. strain W83) of P. gingivalis can be found in certain periodontitis patients.
牙龈卟啉单胞菌被认为是牙周疾病中的一种重要病原体。虽然该微生物表达多种毒力因子，但迄今为止，尚未有结合不同毒力多态性的研究。本研究调查了62株来自患有慢性牙周炎或牙周脓肿患者的牙龈卟啉单胞菌中联合毒力（Cv）基因型的发生情况。基于菌毛（fimA）、赖氨酸特异性半胱氨酸蛋白酶（kgp）和精氨酸特异性半胱氨酸蛋白酶（prpR1/rgpA）的基因变异，通过聚合酶链反应（PCR）对Cv基因型进行评估。分离株还进行了荚膜多糖K-血清分型。共鉴定出基于fimA:kgp:rgpA的18种Cv基因型变体，其中II:I:A和II:II:A Cv基因型（53.3%）是两种最常检测到的组合。此外，36%的分离株可进行K-分型，K6血清型最为常见（23%）。有两株分离株与强毒株W83具有相同的基因型。结果表明，慢性牙周炎与特定的强毒克隆类型无关。在某些牙周炎患者中可发现高毒力基因型（如菌株W83）的牙龈卟啉单胞菌。
