Yoshino T, Laine M L, van Winkelhoff A J, Dahlén G
Department of Oral Microbiology, Institute of Odontology, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Oral Microbiol Immunol. 2007 Jun;22(3):195-200. doi: 10.1111/j.1399-302X.2007.00350.x.
A significant genetic polymorphism has been shown for Porphyromonas gingivalis isolates from different geographical areas. It is, however, possible that genetic similarities can be found among isolates obtained from a more specific population. The aim of the present study was to evaluate genetic heterogeneity among P. gingivalis isolates obtained from Swedish subjects with chronic periodontitis and from periodontal abscess lesions.
A total of 78 P. gingivalis strains, including 55 fresh clinical isolates obtained from 52 Swedish periodontitis subjects, eight isolates from eight Swedish periodontal abscess subjects and 15 reference strains, were subjected to amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) genotyping assays.
A total of 62 AFLP genotypes and 70 RAPD genotypes were identified among the 78 P. gingivalis strains. Forty-six strains were clustered at 70% similarity level into 15 clusters. Six identical RAPD genotypes were identified among the strains. The AFLP/RAPD profiles were compared for identical genotypes. A total of 56 AFLP/RAPD genotypes were found. Four pairs of identical AFLP/RAPD genotypes were found for two strains obtained from two different periodontal pockets each of four subjects. Interestingly, two strains showed an RAPD/AFLP genotype, which was identical to the type strain W83.
The present study demonstrated that Swedish P. gingivalis isolates exhibit a wide variety of genotypes with only a weak clustering pattern. No predominant genotype at the whole chromosomal DNA level was present among Swedish P. gingivalis strains.
已表明来自不同地理区域的牙龈卟啉单胞菌分离株存在显著的基因多态性。然而，从更特定人群中获得的分离株之间有可能发现基因相似性。本研究的目的是评估从患有慢性牙周炎的瑞典受试者和牙周脓肿病变中获得的牙龈卟啉单胞菌分离株之间的基因异质性。
总共78株牙龈卟啉单胞菌菌株，包括从52名瑞典牙周炎受试者中获得的55株新鲜临床分离株、从8名瑞典牙周脓肿受试者中获得的8株分离株以及15株参考菌株，接受扩增片段长度多态性（AFLP）和随机扩增多态性DNA（RAPD）基因分型检测。
在78株牙龈卟啉单胞菌菌株中总共鉴定出62种AFLP基因型和70种RAPD基因型。46株菌株在70%相似性水平上聚为15个簇。在这些菌株中鉴定出6种相同的RAPD基因型。对相同基因型的AFLP/RAPD图谱进行了比较。总共发现了56种AFLP/RAPD基因型。在分别来自4名受试者的两个不同牙周袋中获得的两株菌株中发现了4对相同的AFLP/RAPD基因型。有趣的是，两株菌株显示出一种RAPD/AFLP基因型，与模式菌株W83相同。
本研究表明瑞典牙龈卟啉单胞菌分离株表现出多种基因型，聚类模式较弱。瑞典牙龈卟啉单胞菌菌株中不存在全染色体DNA水平上的优势基因型。
