Peng Qian, Jiang Chongqiu
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
J Fluoresc. 2007 May;17(3):339-43. doi: 10.1007/s10895-007-0177-1. Epub 2007 Apr 18.
A new spectrofluorimetric method was developed for the determination of trace amount of 5-hydroxytryptamine (5-HT) in human urine and serum samples. In the NaAc-HAc buffer solution of pH=5.80, 5-HT can react with formaldehyde-acetylacetone system to form a new compound which sends yellow green fluorescence at 533 nm and the enhanced fluorescence intensity is in proportion to the concentration of 5-HT. Optimum conditions for the determination of 5-HT were also investigated. The dynamic range and detection limit for the determination of 5-HT are 5.35 x 10(-7) approximately 1.07 x 10(-4) mol/L and 2.08 x 10(-7) mol/L, respectively. The developed method is simple, practical and can be successfully applied to determination of 5-HT in human urine and serum samples. Moreover, the enhancement mechanisms of the fluorescence intensity in the 5-HT - formaldehyde-acetylacetone system have been also discussed.
建立了一种新的荧光分光光度法，用于测定人尿液和血清样品中的痕量5-羟色胺（5-HT）。在pH = 5.80的NaAc-HAc缓冲溶液中，5-HT能与甲醛-乙酰丙酮体系反应形成一种新化合物，该化合物在533 nm处发出黄绿色荧光，且荧光强度增强与5-HT浓度成正比。还研究了测定5-HT的最佳条件。测定5-HT的动态范围和检测限分别为5.35×10⁻⁷～1.07×10⁻⁴ mol/L和2.08×10⁻⁷ mol/L。所建立的方法简单、实用，可成功应用于人尿液和血清样品中5-HT的测定。此外，还讨论了5-HT-甲醛-乙酰丙酮体系中荧光强度增强的机制。
