Best S A, Midgley J M, Huang W, Watson D G
Department of Pharmaceutical Sciences, University of Strathclyde, Royal College, Glasgow, UK.
J Pharm Biomed Anal. 1993 Apr-May;11(4-5):323-33. doi: 10.1016/0731-7085(93)80024-u.
Methods were developed for the analysis of 5-hydroxytryptamine, related indolealkylamines (tryptamine, melatonin, 5-methoxytryptamine, N-acetyl-5-hydroxytryptamine and 6-hydroxymelatonin) and 5-hydroxyindole-3-acetic acid (5HIAA) in bovine retina, aqueous and vitreous humours. 5-Hydroxytryptamine and related indolealkylamines were extracted and derivatized to form their corresponding pentafluoropropionyl spirocyclic derivatives. 5HIAA was extracted and derivatized to the corresponding pentafluoropropionamide-trifluoroethyl derivative. Identification and quantitation by gas chromatography-negative ion chemical ionization mass spectrometry was made with reference to deuteriated internal standards. 5-Hydroxytryptamine was present in all (n = 34) retinal samples analysed (20.53 +/- 1.64 ng) while N-acetyl-5-hydroxytryptamine was detected in half of the samples of retina (0.06 +/- 0.02 ng). Melatonin (0.15 +/- 0.06 ng) and tryptamine (0.78 +/- 0.34 ng) were detected in only a small number of retinas. 5-Methoxytryptamine was not present in retina. 5-Hydroxytryptamine was also present in aqueous (0.76 +/- 0.17 ng ml-1 and vitreous (0.35 +/- 0.05 ng ml-1' humours from bovine eye. Tryptamine, melatonin, 5-methoxytryptamine and N-acetyl-5-hydroxytryptamine were not detected in bovine aqueous and vitreous humours. 5HIAA was found in both bovine aqueous (2.03 +/- 0.38 ng ml-1) and vitreous (0.65 +/- 0.06 ng ml-1) humours, but its consistent determination in retina was obviated by interference from spurious peaks.
已开发出用于分析牛视网膜、房水和玻璃体液中5-羟色胺、相关吲哚烷基胺（色胺、褪黑素、5-甲氧基色胺、N-乙酰-5-羟色胺和6-羟基褪黑素）以及5-羟基吲哚-3-乙酸（5HIAA）的方法。5-羟色胺和相关吲哚烷基胺被提取并衍生化，形成它们相应的五氟丙酰基螺环衍生物。5HIAA被提取并衍生化为相应的五氟丙酰胺-三氟乙基衍生物。通过气相色谱-负离子化学电离质谱法进行鉴定和定量，并参照氘代内标物。在所分析的所有（n = 34）视网膜样本中均存在5-羟色胺（20.53±1.64 ng），而在一半的视网膜样本中检测到N-乙酰-5-羟色胺（0.06±0.02 ng）。仅在少数视网膜中检测到褪黑素（0.15±0.06 ng）和色胺（0.78±0.34 ng）。视网膜中不存在5-甲氧基色胺。5-羟色胺也存在于牛眼的房水（0.76±0.17 ng ml-1）和玻璃体液（0.35±0.05 ng ml-1）中。在牛房水和玻璃体液中未检测到色胺、褪黑素、5-甲氧基色胺和N-乙酰-5-羟色胺。在牛房水（2.03±0.38 ng ml-1）和玻璃体液（0.65±0.06 ng ml-1）中均发现了5HIAA，但由于假峰的干扰，无法在视网膜中持续测定其含量。
