Simões Lúcia Chaves, Simões Manuel, Oliveira Rosário, Vieira Maria João
Centro de Engenharia Biológica, Universidade do Minho, Braga, Portugal.
J Basic Microbiol. 2007 Apr;47(2):174-83. doi: 10.1002/jobm.200610224.
Heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rDNA gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity (contact angle measurements) and ii. by measuring adherence to eight different substrata (ASI 304 and 316 stainless steel, copper, polyvinyl chloride, polypropylene, polyethylene, silicone and glass). All the test organisms were hydrophilic and inter-species variation in hydrophobicity occurred only for Comamonas acidovorans. Stainless steel 304 (SS 304), copper, polypropylene (PP), polyethylene (PE) and silicone thermodynamically favoured adhesion for the majority of test strains (>18/25), whilst adhesion was generally less thermodynamically favorable for stainless steel 316 (SS 316), polyvinyl chloride (PVC) and glass. The predictability of thermodynamic adhesion test methods was validated by comparison with 24-well microtiter plate assays using nine reference strains and three adhesion surfaces (SS 316, PVC and PE). Results for Acinetobacter calcoaceticus, Burkolderia cepacia and Stenotrophomonas maltophilia sp. 2 were congruent between both methods whilst they differed for the other bacteria to at least one material. Only A. calcoaceticus had strongly adherent properties to the three tested surfaces. Strain variation in adhesion ability was detected only for Sphingomonas capsulata. Analysis of adhesion demonstrated that in addition to physicochemical surface properties of bacterium and substratum, biological factors are involved in early adhesion processes, suggesting that reliance on thermodynamic approaches alone may not accurately predict adhesion capacity.
从饮用水中分离出了异养细菌（11个属、14个种、25个推定菌株），通过生化方法或16S rDNA基因部分测序进行鉴定，并通过两种方法测定其黏附特性：i. 通过测量疏水性（接触角测量）对黏附潜力进行热力学预测；ii. 通过测量对八种不同基质（304和316不锈钢、铜、聚氯乙烯、聚丙烯、聚乙烯、硅酮和玻璃）的黏附情况。所有测试微生物均为亲水性，仅嗜酸丛毛单胞菌存在种间疏水性差异。对于大多数测试菌株（>18/25），304不锈钢（SS 304）、铜、聚丙烯（PP）、聚乙烯（PE）和硅酮在热力学上有利于黏附，而对于316不锈钢（SS 316）、聚氯乙烯（PVC）和玻璃，黏附在热力学上通常不太有利。通过与使用9种参考菌株和3种黏附表面（SS 316、PVC和PE）的24孔微量滴定板试验进行比较，验证了热力学黏附测试方法的可预测性。乙酸钙不动杆菌、洋葱伯克霍尔德菌和嗜麦芽窄食单胞菌sp. 2的两种方法结果一致，而其他细菌在至少一种材料上的结果不同。只有乙酸钙不动杆菌对三种测试表面具有强黏附特性。仅在荚膜鞘氨醇单胞菌中检测到黏附能力的菌株差异。黏附分析表明，除了细菌和基质的物理化学表面特性外，生物因素也参与早期黏附过程，这表明仅依靠热力学方法可能无法准确预测黏附能力。
