Jestoi Marika, Rokka Mervi, Peltonen Kimmo
Finnish Food Safety Authority (Evira), Department of Animal Diseases and Food Safety Research, Chemistry and Toxicology Unit, Helsinki, Finland.
Mol Nutr Food Res. 2007 May;51(5):625-37. doi: 10.1002/mnfr.200600232.
The usefulness of an existing sample preparation technique used for ionophoric coccidiostats (lasalocid, monensin, salinomycin and narasin) was applied in the analysis of emerging Fusarium-mycotoxins beauvericin (BEA) and enniatins (ENNs) in poultry tissues (liver and meat). Also, maduramicin and liver as a new sample matrix was introduced. The developed methods were validated and applied for the determination of coccidiostats and BEA/ENNs in Finnish poultry tissues in 2004-2005. The validation parameters demonstrated that the integrated sample preparation technique is applicable to the parallel determination of these contaminants in poultry tissues. Of the samples analysed (276 meat and 43 liver), only trace levels of LAS, MON, SAL, NAR and MAD were detected in 7, 3, 5, 6 and 4% of the samples, respectively. Interestingly, for the first time, traces of BEA and ENNs could also be detected in animal tissues. BEA and ENNs A, A1, B and B1 were found in 2, 0.3, 0.6, 4 and 3% of the samples, respectively. The simultaneous presence of coccidiostats and mycotoxins was detected in three turkey samples in 2004.
一种用于离子载体类抗球虫药（拉沙洛西、莫能菌素、盐霉素和那拉菌素）的现有样品制备技术被用于分析家禽组织（肝脏和肌肉）中新兴的镰刀菌属霉菌毒素——白僵菌素（BEA）和恩镰孢菌素（ENNs）。此外，还引入了马杜霉素和肝脏作为新的样品基质。所开发的方法经过验证，并应用于2004 - 2005年芬兰家禽组织中抗球虫药和BEA/ENNs的测定。验证参数表明，该集成样品制备技术适用于家禽组织中这些污染物的平行测定。在所分析的样品中（276份肌肉和43份肝脏），分别仅在7%、3%、5%、6%和4%的样品中检测到痕量水平的拉沙洛西（LAS）、莫能菌素（MON）、盐霉素（SAL）、那拉菌素（NAR）和马杜霉素（MAD）。有趣的是，首次在动物组织中也检测到了痕量的BEA和ENNs。分别在2%、0.3%、0.6%、4%和3%的样品中发现了BEA以及ENNs A、A1、B和B1。2004年在三个火鸡样品中检测到抗球虫药和霉菌毒素同时存在。
