Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Toxins (Basel). 2018 Jan 18;10(1):45. doi: 10.3390/toxins10010045.
The mycotoxin enniatin B (ENN B) is a so-called emerging mycotoxin frequently contaminating poultry feed. To investigate the impact of chronic ENN B exposure on animal health, broiler chickens were fed either a diet naturally contaminated with ENN B (2352 µg/kg) or a control diet (135 µg/kg) for 2, 7, 14, or 21 days. ENN B concentrations were determined in plasma and liver using a validated ultra-high performance liquid chromatography-tandem mass spectrometry UHPLC-MS/MS method. Liver was evaluated histologically, and the villus length and crypt depth of the duodenum, jejunum, and ileum were measured. Histopathology of the livers did not reveal major abnormalities. Feeding an ENN B-contaminated diet could possibly inhibit the proliferation of enterocytes in the duodenal crypts, but did not affect villus length, crypt depth, or villus length-crypt depth ratio of the jejunum and ileum. ENN B levels in plasma and liver were significantly higher in the ENN B-fed group and ranged between <25-264 pg/mL and <0.05-0.85 ng/g, respectively. ENN B carry-over rates from feed to liver tissue were 0.005-0.014% and 0.034-0.109% in the ENN B and control group, respectively. Carry-over rates were low and indicated a limited contribution of poultry tissue-derived products to the total dietary ENN B intake for humans. The above results support the opinion of the European Food Safety Authority stating that adverse health effects from ENN B in broiler chickens are unlikely.
真菌毒素恩镰菌素 B（ENN B）是一种常见的新兴真菌毒素，经常污染家禽饲料。为了研究慢性 ENN B 暴露对动物健康的影响，研究人员用天然污染 ENN B（2352 µg/kg）的饮食或对照饮食（135 µg/kg）喂养肉鸡 2、7、14 或 21 天。采用经验证的超高效液相色谱-串联质谱法（UHPLC-MS/MS）测定血浆和肝脏中的 ENN B 浓度。对肝脏进行组织学评估，并测量十二指肠、空肠和回肠的绒毛长度和隐窝深度。肝脏的组织病理学未显示出主要异常。用 ENN B 污染的饮食喂养可能会抑制十二指肠隐窝中的肠细胞增殖，但不会影响空肠、回肠的绒毛长度、隐窝深度或绒毛长度-隐窝深度比值。在 ENN B 喂养组中，血浆和肝脏中的 ENN B 水平显著升高，分别为<25-264 pg/mL 和<0.05-0.85 ng/g。从饲料到肝脏组织的 ENN B 转移率在 ENN B 组和对照组分别为 0.005-0.014%和 0.034-0.109%。转移率较低，表明禽肉组织产品对人类总膳食 ENN B 摄入量的贡献有限。上述结果支持欧洲食品安全局的观点，即肉鸡中 ENN B 不会产生不良健康影响。
