Rittenberg T, Longaker M T, Adzick N S, Ehrlich H P
Wound Healing Laboratory, Shriners Burns Institute, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
J Cell Physiol. 1991 Dec;149(3):444-50. doi: 10.1002/jcp.1041490313.
Sutured incisional wounds made in fetal sheep and rabbits heal without scarring. Fetal sheep excisional wounds can close by contraction, but those in fetal rabbits do not. In vivo and in vitro evidence suggests that rabbit amniotic fluid inhibits wound contraction. The question arises: does sheep amniotic fluid promote wound contraction because their fetal wounds close by contraction? Sheep amniotic fluid (SAF) from 100 and 125 days gestation was tested in fibroblast populated collagen lattice (FPCL) system, an in vitro model of wound contraction. SAF stimulated FPCL contraction in a dose responsive manner. SAF from a 100 day fetus was more stimulating than a 125 day SAF. SAF enhanced FPCL contraction in the presence or absence of serum. SAF was fractionated by size, using column chromatography. It yielded a fraction with an estimated molecular weigh near 40,000 daltons, which stimulated FPCL contraction. The factor was inactivated by proteolytic digestion and heat denaturation. This protein fraction which stimulates FPCL contraction is not related to 1) actin-myosin filaments enhanced contraction by ATP-induced cell contraction, 2) promotion of fibroblast elongation on glass surface or in collagen, or 3) increased cell number by enhanced fibroblast duplication in a collagen matrix. A mechanism for SAF promotion of FPCL contraction was investigated but not identified.
在胎羊和兔子身上制造的缝合切口伤口能够无痕愈合。胎羊的切除伤口可通过收缩闭合，但胎兔的伤口则不然。体内和体外证据表明，兔羊水会抑制伤口收缩。问题由此产生：羊羊水是否会促进伤口收缩，因为它们的胎儿伤口是通过收缩闭合的？在成纤维细胞填充胶原晶格（FPCL）系统（一种伤口收缩的体外模型）中测试了妊娠100天和125天的羊羊水（SAF）。SAF以剂量反应方式刺激FPCL收缩。来自100天胎儿的SAF比125天的SAF更具刺激性。无论有无血清，SAF均可增强FPCL收缩。使用柱色谱法按大小对SAF进行分级分离。得到一个估计分子量接近40,000道尔顿的级分，该级分可刺激FPCL收缩。该因子通过蛋白水解消化和热变性失活。这种刺激FPCL收缩的蛋白质级分与以下方面无关：1）肌动蛋白 - 肌球蛋白丝通过ATP诱导的细胞收缩增强收缩；2）促进成纤维细胞在玻璃表面或胶原中的伸长；3）通过胶原基质中增强的成纤维细胞复制增加细胞数量。对SAF促进FPCL收缩的机制进行了研究，但尚未确定。
