Pessi A, Bianchi E, Chiappinelli L, Nardi A, Fanali S
Peptide Synthesis Unit, Sclavo SpA, Rome, Italy.
J Chromatogr. 1991 Sep 20;557(1-2):307-13. doi: 10.1016/s0021-9673(01)87140-5.
Capillary zone electrophoresis (CZE) was applied to the analysis of a recently described class of synthetic branched peptides, multiple antigen peptides (MAPs). In comparison with high-performance liquid chromatography (HPLC), CZE showed superior resolution of minor impurities in samples of MAPs obtained by preparative chromatography. MAPs that differed only in the substitution of uncharged residues (Ala-Ser) could be separated by the addition of organic solvents (acetonitrile, methanol, ethanol) to the aqueous background electrolyte.
毛细管区带电泳（CZE）被应用于分析一类最近描述的合成支链肽，即多抗原肽（MAPs）。与高效液相色谱（HPLC）相比，CZE在通过制备色谱法获得的MAPs样品中对微量杂质具有更高的分离度。仅在不带电残基（丙氨酸-丝氨酸）取代方面存在差异的MAPs可以通过向水性背景电解质中添加有机溶剂（乙腈、甲醇、乙醇）来分离。
