Yu Min, Wang Xing-xiang, Zhang Fu-rong, Shang Yun-peng, Du Yu-xi, Chen Hong-juan, Chen Jun-zhu
Department of Cardiovascular Disease, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
J Zhejiang Univ Sci B. 2007 Apr;8(4):221-7. doi: 10.1631/jzus.2007.B0221.
Idiopathic pulmonary arterial hypertension (IPAH) is a rare disease of unknown etiology. The exact pathogenesis of pulmonary arterial hypertension is still not well known. In the past decades, many protein molecules have been found to be involved in the development of IPAH. With proteomic techniques, profiling of human plasma proteome becomes more feasible in searching for disease-related markers. In present study, we showed the protein expression profiles of the serum of IPAH and healthy controls after depleting a few high-abundant proteins in serum. Thirteen spots had changed significantly in IPAH compared with healthy controls and were identified by LC-MS/MS. Alpha-1-antitrypsin and vitronectin were down-regulated in IPAH and may be valuable candidates for further explorations of their roles in the development of IPAH.
特发性肺动脉高压（IPAH）是一种病因不明的罕见疾病。肺动脉高压的确切发病机制仍不清楚。在过去几十年中，已发现许多蛋白质分子参与IPAH的发展。随着蛋白质组学技术的发展，在寻找疾病相关标志物方面，对人类血浆蛋白质组进行分析变得更加可行。在本研究中，我们展示了在去除血清中一些高丰度蛋白质后，IPAH患者和健康对照者血清的蛋白质表达谱。与健康对照相比，IPAH中有13个斑点发生了显著变化，并通过液相色谱-串联质谱（LC-MS/MS）进行了鉴定。α-1-抗胰蛋白酶和玻连蛋白在IPAH中表达下调，可能是进一步探索它们在IPAH发展中作用的有价值候选物。
