Helwig U, Rubin D, Kiosz J, Bitter W, Schreiber S, Döring F, Fölsch U R, Schrezenmeir J
Federal Research Centre for Nutrition and Food, Location Kiel, Kiel, Germany.
Horm Metab Res. 2007 Apr;39(4):237-43. doi: 10.1055/s-2007-972578.
The fatty acid binding protein 2 (FABP2) mediates the intestinal uptake of fatty acids. We and others have identified six FABP2 promoter polymorphisms which result in two haplotypes, A and B. Reporter-gene assays indicated different activity in FABP2 promoter alleles A and B and different responsiveness to PPAR ligands. IN SILICO analysis revealed different putative binding sites in FABP2 haplotypes for retinoid-dependent transcription factors. Therefore, we assumed that retinol supplementation may effect postprandial fat uptake differently in men with FABP2 promoter haplotype A and B. To test this hypothesis, we administered 5000 I.U. retinol/day for 8 weeks to 19 homozygotes for AA and 21 homozygotes for BB and assessed the alteration of postprandial triglycerides during this intervention. FABP2 genotype groups did not significantly differ in anthropometric and laboratory parameters. The alteration of postprandial triglycerides did not differ significantly between genotypes during intervention. This also held true after adjustment for BMI. Furthermore, in a subgroup which had a combination of promoter and common exon polymorphism, the alteration of the postprandial triglycerides did not differ between genotypes. In conclusion, the postprandial triglyceride metabolism of FABP2 promoter AA and BB did not respond differently to retinol administration even though IN SILICO analysis suggested this.
脂肪酸结合蛋白2（FABP2）介导肠道对脂肪酸的摄取。我们和其他人已经鉴定出六种FABP2启动子多态性，这些多态性导致两种单倍型，即A和B。报告基因检测表明FABP2启动子等位基因A和B具有不同的活性，并且对PPAR配体具有不同的反应性。计算机分析揭示了FABP2单倍型中类视黄醇依赖性转录因子的不同假定结合位点。因此，我们推测补充视黄醇可能对具有FABP2启动子单倍型A和B的男性餐后脂肪摄取产生不同的影响。为了验证这一假设，我们给19名AA纯合子和21名BB纯合子每天服用5000国际单位视黄醇，持续8周，并评估了在此干预期间餐后甘油三酯的变化。FABP2基因型组在人体测量和实验室参数方面没有显著差异。干预期间，各基因型之间餐后甘油三酯的变化没有显著差异。在调整BMI后也是如此。此外，在一个具有启动子和常见外显子多态性组合的亚组中，各基因型之间餐后甘油三酯的变化没有差异。总之，尽管计算机分析表明FABP2启动子AA和BB的餐后甘油三酯代谢对视黄醇给药的反应不同，但实际上并非如此。
