Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Physiology and Biochemistry of Nutrition, Kiel, Karlsruhe, Germany.
J Nutr. 2010 Aug;140(8):1411-7. doi: 10.3945/jn.109.118034. Epub 2010 Jun 9.
The intestinal fatty acid binding protein (FABP2) is involved in lipid metabolism whereby variations in the promoter (haplotypes A/B) and exon 2 (Ala54Thr) are associated with dyslipidemia and insulin resistance. To elucidate which factors determine FABP2 expression in human mucosa, we investigated the association between fat intake, genotypes, biochemical variables, and FABP2 expression. FABP2 gene expression was assessed in duodenal specimens from 100 participants who answered a FFQ and who were genotyped and characterized for traits of metabolic syndrome and further biochemical data. Homozygotes for haplotype A tended to have lower fat intake than B-allele carriers (P = 0.066). Searching for an explanation, we evaluated the orexigenic glucose-dependent insulinotropic polypeptide (GIP) in a subset from the Metabolic Intervention Cohort Kiel. AA homozygotes had lower postprandial GIP concentrations than BB homozygotes. Duodenal FABP2 expression was correlated with (n-3) fatty acid (FA) intake in AA homozygotes (r = 0.49; P = 0.021). It was higher in AA homozygotes than in B-allele carriers after adjustment for (n-3) FA intake (P = 0.049) and was negatively correlated with serum FFA (r = -0.41; P < 0.01). Our data indicate that FABP2 expression depends on (n-3) FA intake and FABP2 genotypes. FABP2 might be involved in regulating food intake and intestinal FA utilization.
肠脂肪酸结合蛋白（FABP2）参与脂质代谢，启动子（单倍型 A/B）和外显子 2（Ala54Thr）的变异与血脂异常和胰岛素抵抗有关。为了阐明哪些因素决定了人类黏膜中 FABP2 的表达，我们研究了脂肪摄入、基因型、生化变量和 FABP2 表达之间的关系。我们评估了 100 名参与者的十二指肠标本中的 FABP2 基因表达，这些参与者回答了一份食物频率问卷，并进行了基因分型和代谢综合征特征以及进一步的生化数据描述。单倍型 A 的纯合子比 B 等位基因携带者的脂肪摄入量低（P = 0.066）。为了寻找解释，我们在基尔代谢干预队列中对一部分人进行了葡萄糖依赖性胰岛素释放肽（GIP）的评估。AA 纯合子的餐后 GIP 浓度低于 BB 纯合子。十二指肠 FABP2 表达与 AA 纯合子的（n-3）脂肪酸（FA）摄入呈正相关（r = 0.49；P = 0.021）。调整（n-3）FA 摄入后，AA 纯合子的 FABP2 表达高于 B 等位基因携带者（P = 0.049），并且与血清游离脂肪酸（FFA）呈负相关（r = -0.41；P < 0.01）。我们的数据表明，FABP2 的表达取决于（n-3）FA 的摄入和 FABP2 的基因型。FABP2 可能参与调节食物摄入和肠道 FA 的利用。
