首次在母乳中检测到抗诺如病毒的IgA。

First detection of IgA against norovirus in breast milk.

作者信息

Makita Kohsuke, Hayakawa Yuko, Okame Michio, Homma Kazuhiro, Phan Tung Gia, Okitsu Shoko, Ushijima Hiroshi

机构信息

Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

出版信息

Clin Lab. 2007;53(3-4):125-8.

PMID:17447646

Abstract

Breast milk samples (n = 239) collected from 1994-1997 from mothers at Chiba City, Japan, were tested for antinorovirus antibody by ELISA and western blot methods. It was found that 31 breast milk samples contained IgA against norovirus and this represented 13%. Breast milk could react with a diversity of norovirus genotypes. The highest number of samples containing IgA against norovirus was found in genotype GII/6 (11.3%) and the lowest in GI/8, GII/8 and GII/12 (each of 0.8%). Of note, twenty-eight samples showed reactivity to more than one different norovirus genotypes. Interestingly, three samples demonstrated cross-reaction with both norovirus genogroups I and II. This report is noteworthy because it is the first, to the best of our knowledge, demonstrating the presence of antibody against norovirus in breast milk.

摘要

1994年至1997年期间，从日本千叶市的母亲那里收集了239份母乳样本，采用酶联免疫吸附测定（ELISA）和蛋白质印迹法检测其中的抗诺如病毒抗体。结果发现，31份母乳样本含有抗诺如病毒的IgA，占比13%。母乳可与多种诺如病毒基因型发生反应。含有抗诺如病毒IgA的样本中，数量最多的是GII/6基因型（11.3%），最少的是GI/8、GII/8和GII/12基因型（均为0.8%）。值得注意的是，有28份样本对不止一种不同的诺如病毒基因型有反应。有趣的是，有3份样本与诺如病毒基因组I和II都发生了交叉反应。据我们所知，本报告具有重要意义，因为它首次证明了母乳中存在抗诺如病毒抗体。