Siqueira Walter L, Zhang Weimin, Helmerhorst Eva J, Gygi Steven P, Oppenheim Frank G
Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, Boston, Massachusetts 02118, USA.
J Proteome Res. 2007 Jun;6(6):2152-60. doi: 10.1021/pr060580k. Epub 2007 Apr 21.
The acquired enamel pellicle is a thin protein film forming upon exposure of tooth enamel surfaces to saliva. The structural analysis of this integument relies on efficient pellicle harvesting and protein identification procedures. Material from three individual subjects and two pooled samples yielded the identification by LC-ESI-MS/MS of 130 pellicle proteins of which 89 were found in three or more experiments. A high intersubject consistency in pellicle composition was observed.
获得性釉质 pellicle 是牙釉质表面暴露于唾液时形成的一层薄蛋白质膜。对这种覆盖物的结构分析依赖于高效的 pellicle 采集和蛋白质鉴定程序。来自三个个体受试者和两个混合样本的材料通过 LC-ESI-MS/MS 鉴定出 130 种 pellicle 蛋白，其中 89 种在三个或更多实验中被发现。观察到 pellicle 组成在受试者之间具有高度一致性。
