Mutlu S, Matthews J B, Midda M, Scully C, Prime S S
Department of Oral Medicine, University of Bristol, U.K.
J Pathol. 1991 Oct;165(2):129-36. doi: 10.1002/path.1711650208.
This study quantified the constitutive and interferon-gamma (IFN-gamma) stimulated expression of MHC class I (HLA-ABC and beta 2 microglobulin) and class II antigens (HLA-DR, -DP, -DQ) on normal and malignant oral keratinocytes using radioimmunoassay and immunocytochemical techniques. Normal keratinocytes and three of four malignant cell lines (H103, H157, H314) expressed MHC class I antigens constitutively; IFN-gamma increased MHC class I expression with significant changes in normals, H157 and H314. Normal keratinocytes expressed significantly more constitutive MHC class I antigens than H103 and H157 and significantly more IFN-gamma stimulated MHC class I antigens than H103, H157 and H314. MHC class II antigens predominantly were not expressed constitutively on normals, H103 and H157 but, in H314, HLA-DR, -DP and -DQ antigens were demonstrated on 35, 11 and 5 per cent of cells, respectively, and resulted in a non-coordinated pattern of expression (HLA-DR greater than -DP = -DQ). IFN-gamma induced HLA-DR on normals, H103 and H157, whilst HLA-DP and -DQ remained undetectable. In H314, IFN-gamma enhanced HLA-DR, -DP and -DQ (significant increase of HLA-DQ) but the interrelationship between these antigens was maintained (HLA-DR greater than -DP = -DQ). Normal keratinocytes expressed significantly more IFN-gamma stimulated HLA-DR than H103 and H157 but significantly less HLA-DR than H314 under similar experimental conditions. One oral malignant cell line (H191) did not express MHC class I and MHC class II antigens either constitutively or in response to IFN-gamma. The results demonstrate aberrant patterns of MHC expression (absence, enhanced, diminished) in the different malignant oral keratinocyte cell lines.
本研究采用放射免疫测定法和免疫细胞化学技术，对正常和恶性口腔角质形成细胞中主要组织相容性复合体I类（HLA - ABC和β2微球蛋白）和II类抗原（HLA - DR、- DP、- DQ）的组成性表达以及γ干扰素（IFN - γ）刺激后的表达进行了定量分析。正常角质形成细胞和四个恶性细胞系中的三个（H103、H157、H314）组成性表达MHC I类抗原；IFN - γ增加了MHC I类抗原的表达，在正常细胞、H157和H314中变化显著。正常角质形成细胞组成性表达的MHC I类抗原显著多于H103和H157，且IFN - γ刺激后表达的MHC I类抗原显著多于H103、H157和H314。MHC II类抗原在正常细胞、H103和H157中主要不组成性表达，但在H314中，分别有35%、11%和5%的细胞表达HLA - DR、- DP和- DQ抗原，且呈现出非协调性表达模式（HLA - DR＞- DP = - DQ）。IFN - γ诱导正常细胞、H103和H157表达HLA - DR，而HLA - DP和- DQ仍无法检测到。在H314中，IFN - γ增强了HLA - DR、- DP和- DQ的表达（HLA - DQ显著增加），但这些抗原之间的相互关系得以维持（HLA - DR＞- DP = - DQ）。在相似实验条件下，正常角质形成细胞IFN - γ刺激后表达的HLA - DR显著多于H103和H157，但显著少于H314。一个口腔恶性细胞系（H191）无论是组成性表达还是对IFN - γ的反应，均不表达MHC I类和MHC II类抗原。结果表明，不同的恶性口腔角质形成细胞系中存在MHC表达异常模式（缺失、增强、减弱）。
