Ding Juan-Juan, He Qing-Nan, Zhou Pin, Zhang Lei, Yi Zhu-Wen
Department of Pediatrics, Second Xiangya Hospital, Central South University, Changsha, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2007 Apr;9(2):122-4.
To establish a urinary proteomic map on two-dimensional polyacrylamide gelelectrophoresis (2-DE) in children with idiopathic nephrotic syndrome (INS).
The proteins from INS children were purified by four various means, separated by 2-DEand stained by silver.
The sequential preparation of urinary proteins by acetone precipitation and dislysis, when the sample was 300 microg, resulted in a clear background, well-resolved and reproducible 2-DE urinary protemic map in children with INS.
A steady 2-DE technique for urinary protemic map in children with INS was established, which can be effectively applied in urinary proteomics of the disease.
