[外周血单个核细胞二维电泳图谱的建立]
[Establishment of 2-dimensional electrophoresis maps of peripheral blood mononuclear cells].
作者信息
Zhong Bai-Yun, Fan Xue-Gong, Liu Wei-Dong, Yang Yi-Xuan, You Yun-Hui
机构信息
Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Apr;32(2):299-303.
OBJECTIVE
To establish the 2-dimensional electrophoresis(2-DE) profiles of peripheral blood mononuclear cells(PBMC) in patients with hepatocellular carcinoma(HCC) and health adults.
METHODS
The total proteins from PBMC in patients with HCC and healthy adult were separated by immobilized pH gradient-based 2-DE. The differential expression proteins were analyzed by PDQuest analysis software.
RESULTS
The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults were obtained. For HCC, the average spots of 2-DE maps were 1 206 +/- 48, and the average matching rate was 90.8%. For normal adults, the average spots were 1 123 +/- 37, and the average matching rate was 92.6%.
CONCLUSION
The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults are established. These proteomic analysis methods are useful to screen the potential biomarkers in the early diagnosis, treatment and prognosis monitor in patients with malignant tumor.
目的
建立肝细胞癌（HCC）患者和健康成年人外周血单个核细胞（PBMC）的二维电泳（2-DE）图谱。
方法
采用基于固定化pH梯度的二维电泳技术分离HCC患者和健康成年人外周血单个核细胞中的总蛋白。利用PDQuest分析软件分析差异表达蛋白。
结果
获得了HCC患者和健康成年人外周血单个核细胞分辨率良好、可重复的二维电泳图谱。对于HCC患者，二维电泳图谱的平均点数为1206±48，平均匹配率为90.8%。对于正常成年人，平均点数为1123±37，平均匹配率为92.6%。
结论
建立了HCC患者和健康成年人外周血单个核细胞分辨率良好、可重复的二维电泳图谱。这些蛋白质组学分析方法有助于筛选恶性肿瘤患者早期诊断、治疗及预后监测的潜在生物标志物。
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