Sochová Ivana, Hofman Jakub, Holoubek Ivan
RECETOX - Research Centre for Environmental Chemistry and Ecotoxicology, Masaryk University Brno, Kamenice 126/3, Brno, CZ-625 00, Czech Republic.
Environ Int. 2007 Aug;33(6):798-804. doi: 10.1016/j.envint.2007.03.001. Epub 2007 Apr 20.
Caenorhabditis elegans is a free-living soil nematode that is commonly used as a model for toxicity tests. The aim of this study was to investigate the toxicity of seven organic pollutants: four azaarenes (quinoline, acridine, phenazine, and 1,10-phenanthroline), short-chain chlorinated paraffins, and two organochlorinated pesticides (toxaphene and hexachlorobenzene). The exposure to all chemicals was carried out in three test media (soil, agar, and aquatic medium), and adult mortality was evaluated after 24 and 48 h. Toxaphene was the most toxic substance with LC(50) (48 h) of 379 mg/kg in the soil and 0.2 mg/L in the aquatic medium. Quinoline was the most toxic chemical in agar test with LC(50) (48 h) of 10 mg/L. HCB showed a very low toxicity in all tests, maybe due to its very low water solubility. Longer than 24-h test duration was found necessary for getting more correct data on toxicity. In comparison with other studies, C. elegans was less sensitive than other soil invertebrates. Different response might be attributed to different exposure routes and shorter test duration. Equilibrium partitioning theory was used to calculate K(oc) from results of soil and aquatic tests but this approach was found not working. Our results suggest that the tests with nematode C. elegans should be included to the battery of tests for risk assessment of POPs in soil.
秀丽隐杆线虫是一种自由生活的土壤线虫，常用于毒性测试模型。本研究的目的是调查七种有机污染物的毒性：四种氮杂芳烃（喹啉、吖啶、吩嗪和1,10-菲咯啉）、短链氯化石蜡以及两种有机氯农药（毒杀芬和六氯苯）。在三种测试介质（土壤、琼脂和水生介质）中进行所有化学品的暴露实验，并在24小时和48小时后评估成虫死亡率。毒杀芬是毒性最大的物质，在土壤中的LC(50)（48小时）为379毫克/千克，在水生介质中的LC(50)（48小时）为0.2毫克/升。喹啉是琼脂测试中毒性最大的化学品，LC(50)（48小时）为10毫克/升。六氯苯在所有测试中显示出极低的毒性，可能是由于其极低的水溶性。发现需要超过24小时的测试持续时间才能获得更准确的毒性数据。与其他研究相比，秀丽隐杆线虫比其他土壤无脊椎动物敏感性更低。不同的反应可能归因于不同的暴露途径和较短的测试持续时间。使用平衡分配理论根据土壤和水生测试结果计算K(oc)，但发现这种方法不起作用。我们的结果表明，线虫秀丽隐杆线虫测试应纳入持久性有机污染物土壤风险评估的测试组合中。
