Grigoriev Andrei
GPC Biotech, Fraunhoferstrasse 20, Martinsried 82152, Germany.
Nucleic Acids Res. 2003 Jul 15;31(14):4157-61. doi: 10.1093/nar/gkg466.
Using two different approaches, we estimated that on average there are about five interacting partners per protein in the proteome of the yeast Saccharomyces cerevisiae. In the first approach, we used a novel method to model sampling overlap by a Bernoulli process, compared the results of two independent yeast two-hybrid interaction screens and tested the robustness of the estimate. The most stable estimate of five interactors per protein was obtained when the three most highly connected nodes in the protein interaction network were removed from the analysis (eight interactors per protein if those nodes were kept). In the second approach, we analysed a published high-confidence subset of putative interaction data obtained from multiple sources, including large-scale two-hybrid screens, complex purifications, synthetic lethals, correlated gene expression, computational predictions and previous annotations. Strikingly, the estimate was again five interactors per protein. These estimates suggest a range of approximately 16,000-26,000 different interaction pairs in the yeast, excluding homotypic interactions. We also discuss the approaches to estimating the rate of homotypic interactions.
我们采用两种不同方法估计，酿酒酵母蛋白质组中平均每个蛋白质约有五个相互作用伙伴。在第一种方法中，我们使用一种新方法通过伯努利过程对采样重叠进行建模，比较了两个独立的酵母双杂交相互作用筛选结果，并测试了估计值的稳健性。当从分析中去除蛋白质相互作用网络中连接性最高的三个节点时，得到了每个蛋白质五个相互作用伙伴的最稳定估计值（如果保留这些节点，则每个蛋白质有八个相互作用伙伴）。在第二种方法中，我们分析了从多个来源获得的已发表的高可信度推定相互作用数据子集，包括大规模双杂交筛选、复合物纯化、合成致死、相关基因表达、计算预测和先前注释。令人惊讶的是，估计值再次是每个蛋白质五个相互作用伙伴。这些估计表明，在酵母中约有16000 - 26000个不同的相互作用对，不包括同型相互作用。我们还讨论了估计同型相互作用速率的方法。
