Küttel Claudia, Nascimento Elisabete, Demierre Nicolas, Silva Tiago, Braschler Thomas, Renaud Philippe, Oliva Abel G
Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
Acta Trop. 2007 Apr;102(1):63-8. doi: 10.1016/j.actatropica.2007.03.002. Epub 2007 Mar 12.
Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The cellular modifications caused by the intracellular parasite result in a shift in impedance which can be measured dielectrically. Thus, a rapid cell-by-cell detection with microliter amounts of reagents is possible. Unlike other diagnostic tests, this method does not depend on extensive sample pre-treatment or expensive chemicals and equipment.
阻抗谱是用于对活细胞进行无标记分析和表征的强大工具。在这项工作中，我们利用微制造流式细胞仪上的阻抗谱实现了对感染牛巴贝斯虫的红细胞的检测。细胞内寄生虫引起的细胞变化会导致阻抗发生变化，这种变化可以通过介电测量来检测。因此，使用微升量的试剂就可以逐个细胞地进行快速检测。与其他诊断测试不同，该方法不依赖于大量的样品预处理或昂贵的化学试剂和设备。
