Mansor Muhammad Asraf, Ahmad Mohd Ridzuan
Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia.
Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia.
Int J Mol Sci. 2015 Jun 4;16(6):12686-712. doi: 10.3390/ijms160612686.
Electrical properties of living cells have been proven to play significant roles in understanding of various biological activities including disease progression both at the cellular and molecular levels. Since two decades ago, many researchers have developed tools to analyze the cell's electrical states especially in single cell analysis (SCA). In depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis. This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique. This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques. Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.
活细胞的电学性质已被证明在理解包括细胞和分子水平疾病进展在内的各种生物活动中发挥着重要作用。自二十年前以来，许多研究人员开发了工具来分析细胞的电状态，特别是在单细胞分析（SCA）中。只有通过单细胞分析才能完成对细胞分化和癌症活动的深入分析和更全面的描述。与传统的大型技术相比，各种微流控技术的出现支持了这种日益增长的兴趣，这些技术可实现高精度筛选、降低设备成本并缩短用于表征单细胞电学性质的分析时间。本文对单细胞电学性质分析从经典技术到微流控技术最新进展的发展历程进行了综述。重点介绍了不同微流控技术的技术细节，并讨论了各种微流控设备的优缺点。
