Nascimento E, Silva T, Oliva A
Biosensors/Biomolecular Diagnostics Laboratory, Oeiras/Portugal.
Parassitologia. 2007 May;49 Suppl 1:45-52.
Nowadays numerous microfluidic systems are being developed to address a variety of clinical problems. Latest advances in microfluidic technology are promising to revolutionize the detection of pathogens in vivo through the development of integrated lab-on-chip devices. Such microfabricated systems will undertake all steps in sample analysis from collection and preparation to molecular detection. Micro total analysis systems are suitable candidates for point of care diagnostics due to small size, low cost production and enabled portability. The work here presented aimed the use of microfluidic platforms to identify and manipulate bovine red blood cells infected by the protozoan parasite Babesia bovis. A microfabricated device based on impedance spectroscopy was used for single cell discrimination and its sensitivity and applicability as a diagnostic method for bovine babesiosis was studied. Furthermore, manipulation and sorting of normal and infected red blood cells was performed on a dielectrophoresis based microfabricated cell cytometer. Single cell analysis of normal and B. bovis infected red blood cells was performed by electrorotation and dielectric parameters such as permittivities and conductivities of the cellular membrane and cytoplasm were determined.
如今，人们正在开发众多微流控系统以解决各种临床问题。微流控技术的最新进展有望通过开发集成芯片实验室设备彻底改变体内病原体的检测方式。这种微制造系统将承担从样本采集、制备到分子检测的样本分析的所有步骤。微全分析系统由于体积小、生产成本低且便于携带，是即时诊断的合适候选者。本文介绍的工作旨在利用微流控平台识别和操控被原生动物寄生虫牛巴贝斯虫感染的牛红细胞。一种基于阻抗谱的微制造设备用于单细胞鉴别，并研究了其作为牛巴贝斯虫病诊断方法的灵敏度和适用性。此外，在基于介电电泳的微制造细胞流式仪上对正常和感染的红细胞进行了操控和分选。通过电旋转对正常和感染牛巴贝斯虫的红细胞进行单细胞分析，并测定了细胞膜和细胞质的介电常数和电导率等介电参数。
