Dundaroff S, Just I, Falke D
Arch Virol. 1975;49(2-3):117-26. doi: 10.1007/BF01317531.
Simultaneous infection of primary rabbit kidney cells with HSV type 1 TK+ and a TK- strain results in a mutual influence of both viruses on the induction of thymidine kinase (TK). TK+ virus has an enhancing and TK- virus a depressing effect on TK induction by a superinfecting TK+ virus. The enzyme induction depends on the ratio of multiplicities of both viruses. The mutual influence on TK induction depends further on the time of addition of the superinfecting virus: the effect of the second virus can still be observed when given 6 hours after primary infection. Identical phenomena can be observed using combinations with HSV type 2 or Pseudorabies viruses. The ability of HSV to induce TK is progressively inactivated with increasing the time of UV-irradiation. The depressing effect of a TK- strain and the stimulating effect of a TK+ strain on superinfecting TK+ strains is UV-sensitive: after 6 minutes of UV-irradiation neither inhibition nor stimulation of TK induction by a superinfecting TK+ strain can be observed. Infection by long-term (20 minutes) UV-irradiated TK+ strains results in a depression of TK induction by a superinfecting TK+ virus. Long-term irradiation of the TK- virus does not show this effect. Cytosine-arabinoside has no effect on the mutual influence of TK induction by TK+ and TK-strains; the phenomenon of mutual depression therefore has to be considered an early process.
用1型单纯疱疹病毒胸苷激酶阳性（HSV type 1 TK+）毒株和胸苷激酶阴性（TK-）毒株同时感染原代兔肾细胞，会导致两种病毒对胸苷激酶（TK）诱导产生相互影响。TK+病毒对超感染的TK+病毒诱导TK具有增强作用，而TK-病毒则具有抑制作用。酶的诱导取决于两种病毒的感染复数比。对TK诱导的相互影响还取决于超感染病毒的添加时间：初次感染后6小时给予第二种病毒时，仍可观察到其影响。使用2型单纯疱疹病毒或伪狂犬病病毒的组合也可观察到相同现象。随着紫外线照射时间的增加，单纯疱疹病毒诱导TK的能力会逐渐失活。TK-毒株的抑制作用和TK+毒株对超感染的TK+毒株的刺激作用对紫外线敏感：紫外线照射6分钟后，超感染的TK+毒株对TK诱导既无抑制作用也无刺激作用。长期（20分钟）紫外线照射的TK+毒株感染会导致超感染的TK+病毒诱导TK受到抑制。对TK-病毒进行长期照射未显示出这种效果。阿糖胞苷对TK+和TK-毒株诱导TK的相互影响没有作用；因此，相互抑制现象必须被视为一个早期过程。
