Lincopan N, Espíndola N M, Vaz A J, Carmona-Ribeiro A M
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CP 26077, Av. Lineu Prestes 748 - Butantã, CEP 05513-970, São Paulo, SP, Brazil.
Int J Pharm. 2007 Aug 1;340(1-2):216-22. doi: 10.1016/j.ijpharm.2007.03.014. Epub 2007 Mar 12.
Polystyrene sulfate (PSS) particles (301 nm mean diameter) were covered with single cationic dioctadecyldimethylammonium bromide (DDA) bilayers and used for antigen adsorption and presentation. The antigen was a mixture of purified 18/14 Taenia crassiceps proteins (18/14-Tcra). Firstly, the DDA/PSS assembly was characterized at 1mM NaCl and 5 x 10(9) PSS particles/mL over a range of DDA concentrations (0.001-1mM) by means of dynamic light scattering for particle sizing and zeta-potential analysis. 0.01 mM DDA is enough to produce homodisperse and cationic bilayer-covered particles. Secondly, under these experimental conditions, 18/14-Tcra adsorption isotherms onto biomimetic particles or aluminium hydroxide (Al(OH)3) yield limiting adsorption of 0.36 and 1.32 mg protein/mg biomimetic particles or Al(OH)3, respectively. Finally, in mice, superior humoral and cellular immunoresponse from serum IgG and footpad swelling was obtained for antigen/biomimetic particles in comparison to conventional Al(OH)3. Cationic bilayer-covered particles are a novel, highly organized and, possibly, general immunoadjuvant for antigen presentation and subunit vaccine design.
聚苯乙烯磺酸盐（PSS）颗粒（平均直径301纳米）覆盖有单阳离子双十八烷基二甲基溴化铵（DDA）双层膜，用于抗原吸附和呈递。抗原是纯化的18/14克氏绦虫蛋白（18/14-Tcra）的混合物。首先，通过动态光散射进行颗粒大小测定和zeta电位分析，在1mM氯化钠和5×10⁹个PSS颗粒/毫升的条件下，在一系列DDA浓度（0.001 - 1mM）范围内对DDA/PSS组装体进行表征。0.01 mM DDA足以产生均一分散且覆盖有阳离子双层膜的颗粒。其次，在这些实验条件下，18/14-Tcra在仿生颗粒或氢氧化铝（Al(OH)₃）上的吸附等温线分别产生了0.36和1.32毫克蛋白质/毫克仿生颗粒或Al(OH)₃的极限吸附量。最后，在小鼠中，与传统的Al(OH)₃相比，抗原/仿生颗粒在血清IgG和足垫肿胀方面获得了更好的体液和细胞免疫反应。覆盖有阳离子双层膜的颗粒是一种新型的、高度有序的，并且可能是用于抗原呈递和亚单位疫苗设计的通用免疫佐剂。
