Fan Guo-Kang, Wang Hualin, Takenaka Hiroshi
Department of Otorhinolaryngology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Jie-fang Road 88, Hangzhou, Zhejiang 310009, China.
Acta Otolaryngol. 2007 May;127(5):521-6. doi: 10.1080/00016480600951368.
Conclusions. Infiltration and activation of eosinophils is a characteristic of nasal polyposis. Allergic reaction is a risk factor for the accumulation of eosinophils in this disease. T-cell-derived interleukin 5 (IL-5) and autosecretion of IL-5 from activated eosinophils may be causative reasons for the extension and persistence of eosinophil inflammation. Objectives. To investigate whether eosinophils were accumulated and activated in nasal polyposis, and the roles of IL-5, eotaxin, and T cells in this process. Materials and methods. A retrospective study was conducted on 17 tissue samples from patients with nasal polyposis with allergy and 26 cases of non-allergic polyposis. Immunohistochemical staining by specific antibodies was carried out using the alkaline phosphatase anti-alkaline phosphatase method and the avidin-biotin complex technique.Results. The number of EG1-positive cells (pan eosinophil marker) was similar to the number of EG2-positive cells (activated eosinophil marker) in all tissue samples, although EG1- and EG2-positive cells were richer in allergic patients than those in non-allergic patients. Both EG1- and EG2-positive cells were correlated with CD3-positive cells (pan T cell marker) and IL-5-producing cells in allergic or non-allergic polyposis. A large proportion of IL-5 producing cells were eosinophils. Eotaxin protein was detected in all tissue samples and dominantly located in epithelial cells. Eotaxin expression between allergic and non-allergic subjects was not significantly different.
结论。嗜酸性粒细胞的浸润和活化是鼻息肉病的一个特征。过敏反应是该疾病中嗜酸性粒细胞积聚的一个危险因素。T细胞衍生的白细胞介素5（IL-5）以及活化嗜酸性粒细胞的IL-5自分泌可能是嗜酸性粒细胞炎症扩展和持续的原因。目的。研究鼻息肉病中嗜酸性粒细胞是否积聚和活化，以及IL-5、嗜酸性粒细胞趋化因子和T细胞在此过程中的作用。材料与方法。对17例变应性鼻息肉病患者的组织样本和26例非变应性鼻息肉病患者进行回顾性研究。采用碱性磷酸酶抗碱性磷酸酶法和抗生物素蛋白-生物素复合物技术，用特异性抗体进行免疫组织化学染色。结果。在所有组织样本中，EG1阳性细胞（泛嗜酸性粒细胞标志物）的数量与EG2阳性细胞（活化嗜酸性粒细胞标志物）的数量相似，尽管变应性患者中EG1和EG2阳性细胞比非变应性患者中的更丰富。在变应性或非变应性鼻息肉病中，EG1和EG2阳性细胞均与CD3阳性细胞（泛T细胞标志物）和产生IL-5的细胞相关。大部分产生IL-5的细胞是嗜酸性粒细胞。在所有组织样本中均检测到嗜酸性粒细胞趋化因子蛋白，且主要位于上皮细胞中。变应性和非变应性受试者之间的嗜酸性粒细胞趋化因子表达无显著差异。
