Lin Xin-Sheng, Luo Xian-Yang, Wang Hui-Ge, Li Chuang-Wei, Lin Xin, Yan Chu
Department of Otolarynology-Head and Neck Surgery, The Shantou Central Hospital/Affiliated Shantou Hospital of Sun Yet-Sen University, Shantou, Guangdong 515041;
Exp Ther Med. 2013 May;5(5):1476-1480. doi: 10.3892/etm.2013.986. Epub 2013 Mar 1.
The aim of the present study was to investigate the expression, distribution and function of dendritic cells (DCs) and to study their role in nasal polyps. The study involved 55 participants, 45 of whom had nasal polyps and were the study group and 10 who had normal inferior turbinates and were the control group. Immunohistochemical staining was used to visualize the expression and distribution of the S-100 protein. A double immunostaining method was used to visualize the CD1a and CD40 expression and the images were analyzed with Axioplan 2 microscopy. The expression level of the S-100 protein in the nasal polyps was higher than that in the normal inferior turbinates with a significant difference (P<0.01). The distribution area, number and density of the double stained cells in the nasal polyps were all greater than in the normal inferior turbinates (P<0.01). The S-100 protein and double stained cells were mainly located in the lamina propria below the mucous membrane. The present study demonstrates that DCs are involved in the pathogenesis of nasal polyps and the presence of CD40-positive DCs suggests that this was related to the reciprocal interaction between the DCs and T lymphocytes.
本研究的目的是调查树突状细胞（DCs）的表达、分布和功能，并研究它们在鼻息肉中的作用。该研究纳入了55名参与者，其中45名患有鼻息肉，为研究组，10名下鼻甲正常，为对照组。采用免疫组织化学染色来观察S-100蛋白的表达和分布。采用双重免疫染色法观察CD1a和CD40的表达，并使用Axioplan 2显微镜对图像进行分析。鼻息肉中S-100蛋白的表达水平高于正常下鼻甲，差异有统计学意义（P<0.01）。鼻息肉中双重染色细胞的分布面积、数量和密度均大于正常下鼻甲（P<0.01）。S-100蛋白和双重染色细胞主要位于黏膜下方的固有层。本研究表明，DCs参与了鼻息肉的发病机制，CD40阳性DCs的存在表明这与DCs和T淋巴细胞之间的相互作用有关。
