Zhou Xiao-hui, Li Lian-da, Wu Li-mao, Han Lei, Liu Zhong-de, Yang Ji-xiang, Lv Yan-wei, You Chun-lai, Zhou Zhi-heng
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang province, PRC.
Arch Androl. 2007 Mar-Apr;53(2):49-52. doi: 10.1080/01485010600908397.
Male rats were immunized with prostate tissue homogenate supernate (PTHS) of male rats with complete Freund's adjuvant (CFA) intra dermal in the multiple points and simultaneously immunized with 0.5 ml Pertussis-Diphtheria-Tetanus (PDT) vaccine intra peritonea on 0 and 30th day. At the 45th day after first immunization, animals were sacrificed and a series of examinations such as HE stain, assay of TNF-alpha by ELISA and assay of inducible nitric oxide synthase (iNOS) mRNA by in-situ hybridization (ISH) were taken. We observed that there was a remarkable up-regulation of TNF-alpha expression in the high dosage model group. The results of macropathology, histopathology and iNOS ISH also revealed the same tendency. This experimental procedure is effective to induce chronic abacterial prostatitis (CAP).
将雄性大鼠的前列腺组织匀浆上清液（PTHS）与完全弗氏佐剂（CFA）多点皮内注射免疫雄性大鼠，并在第0天和第30天同时腹腔注射0.5 ml百日咳-白喉-破伤风（PDT）疫苗。首次免疫后第45天，处死动物并进行一系列检查，如苏木精-伊红（HE）染色、酶联免疫吸附测定（ELISA）检测肿瘤坏死因子-α（TNF-α）以及原位杂交（ISH）检测诱导型一氧化氮合酶（iNOS）mRNA。我们观察到高剂量模型组中TNF-α表达显著上调。大体病理学、组织病理学和iNOS原位杂交结果也显示出相同趋势。该实验方法可有效诱导慢性非细菌性前列腺炎（CAP）。
