Yan Xing-Ke, Liu An-Guo, He Tian-You, Kan Li-Li, Dong Li-Li, Wang Jun-Yan, Li Hua-Zhang
Department of Acupuncture and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu 730000, China.
Zhonghua Nan Ke Xue. 2013 May;19(5):451-5.
To observe the changes of inflammatory cytokines in autoimmune prostatitis (AIP) rats treated by electro-acupuncture (EA) at Sanyin points.
We selected 40 Wistar male rats in this study, 10 as normal controls, and the other 30 made AIP models by intradermal injection of protein purification liquid from the prostate of allogeneic male rats with dual immune adjuvant. Then we randomly divided the AIP models into a model, a Cernilton control and an EA group of equal number, the latter two groups treated by Cernilton enema and EA, respectively. After 15 days of treatment, all the animals were sacrificed for detection of the levels of TNF-alpha, iNOS, MDA and T-AOC in the prostate tissue.
Compared with the normal controls, the model rats showed significantly elevated TNF-alpha expression ([15.31 +/- 1.36] vs [32.20 +/- 1.65] pg/ml, P < 0.01), iNOS activity ([0.81 +/- 0.33] vs [1.25 +/- 0.23] U/ml, P < 0.01) and MDA content ([0.66 +/- 0.14] vs [0.91 +/- 0.21] nmol/ml, P < 0.05), but markedly reduced T-AOC activity ([1.56 +/- 0.16] vs [1.11 +/- 0.15] U/ml, P < 0.01). In comparison with the model group, the EA group exhibited significantly reduced levels of TNF-alpha ([17.32 +/- 2.69 ] pg/ml, P < 0.01), iNOS ([0.98 +/- 0.5 ] U/ml, P < 0.05) and MDA ([0.70 +/- 0.20] nmol/ml, P < 0.05), but remarkably increased level of T-AOC ([1.44 +/- 0.26] U/ml, P < 0.05).
Electro-acupuncture at Sanyin points can protect the prostate tissue from morphological damage and reduce inflammatory reaction by decreasing pro-inflammatory cytokine activity, vascular permeability and inflammatory cell infiltration and increasing the activity of the antioxidant defense system.
观察三阴交穴位电针治疗自身免疫性前列腺炎（AIP）大鼠后炎症细胞因子的变化。
本研究选取40只Wistar雄性大鼠，10只为正常对照，另外30只采用同种异体雄性大鼠前列腺蛋白纯化液加双重免疫佐剂皮内注射制备AIP模型。然后将AIP模型随机分为模型组、舍尼通对照组和电针组，每组数量相等，后两组分别采用舍尼通灌肠和电针治疗。治疗15天后，处死所有动物，检测前列腺组织中肿瘤坏死因子-α（TNF-α）、诱导型一氧化氮合酶（iNOS）、丙二醛（MDA）水平及总抗氧化能力（T-AOC）。
与正常对照组相比，模型大鼠TNF-α表达水平显著升高（[15.31±1.36]对[32.20±1.65]pg/ml，P<0.01），iNOS活性升高（[0.81±0.33]对[1.25±0.23]U/ml，P<0.01），MDA含量升高（[0.66±0.14]对[0.91±0.21]nmol/ml，P<0.05），但T-AOC活性显著降低（[1.56±0.16]对[1.11±0.15]U/ml，P<0.01）。与模型组相比，电针组TNF-α水平（[17.32±2.69]pg/ml，P<0.01）、iNOS水平（[0.98±0.5]U/ml，P<0.05）和MDA水平（[0.70±0.20]nmol/ml，P<0.05）显著降低，但T-AOC水平显著升高（[1.44±0.26]U/ml，P<0.05）。
三阴交穴位电针可通过降低促炎细胞因子活性、血管通透性和炎症细胞浸润，提高抗氧化防御系统活性，保护前列腺组织免受形态学损伤，减轻炎症反应。
