通过转基因过表达评估八个黑腹果蝇细胞色素P450基因的抗杀虫剂潜力。

Evaluating the insecticide resistance potential of eight Drosophila melanogaster cytochrome P450 genes by transgenic over-expression.

作者信息

Daborn Phillip J, Lumb Christopher, Boey Adrian, Wong Wayn, Ffrench-Constant Richard H, Batterham Philip

机构信息

Centre for Environmental Stress and Adaptation Research (CESAR), Department of Genetics, Bio21 Molecular and Biotechnology Institute, The University of Melbourne, Vic. 3010, Australia.

出版信息

Insect Biochem Mol Biol. 2007 May;37(5):512-9. doi: 10.1016/j.ibmb.2007.02.008. Epub 2007 Mar 2.

DOI:10.1016/j.ibmb.2007.02.008

PMID:17456446

Abstract

In Drosophila melanogaster, the increased expression of Cyp6g1 results in resistance to chemically unrelated insecticides including DDT, neonicotinoids and insect growth regulator insecticides. To determine the insecticide resistance capacity of other D. melanogaster cytochrome P450s, we used the GAL4/UAS system to express individual P450s in the midgut, Malpighian tubules and fat body of transgenic flies. Drosophila over-expressing Cyp6g1, Cyp6g2, Cyp6t3, Cyp6a2, Cyp6a8, Cyp6a19, Cyp6a23 and Cyp12d1 were screened for resistance to four insecticides--DDT, nitenpyram, dicyclanil and diazinon. Increased survival on insecticides is detected for Cyp6g1 (DDT, nitenpyram and dicyclanil), Cyp6g2 (nitenpyram and diazinon) and Cyp12d1 (DDT and dicyclanil) over-expression lines. No increased survival on any insecticide was detected for flies over-expressing either Cyp6a2, Cyp6a8, Cyp6t3, Cyp6a19 or Cyp6a23.

摘要

在黑腹果蝇中，Cyp6g1表达量增加会导致对包括滴滴涕、新烟碱类和昆虫生长调节剂类杀虫剂在内的化学结构不相关的杀虫剂产生抗性。为了确定其他黑腹果蝇细胞色素P450的抗杀虫剂能力，我们使用GAL4/UAS系统在转基因果蝇的中肠、马氏管和脂肪体中表达单个P450。对过表达Cyp6g1、Cyp6g2、Cyp6t3、Cyp6a2、Cyp6a8、Cyp6a19、Cyp6a23和Cyp12d1的果蝇进行了四种杀虫剂——滴滴涕、烯啶虫胺、双甲脒和二嗪农的抗性筛选。在过表达Cyp6g1（滴滴涕、烯啶虫胺和双甲脒）、Cyp6g2（烯啶虫胺和二嗪农）和Cyp12d1（滴滴涕和双甲脒）的品系中检测到对杀虫剂的存活率增加。过表达Cyp6a2、Cyp6a8、Cyp6t3、Cyp6a19或Cyp6a23的果蝇在任何一种杀虫剂上均未检测到存活率增加。