维生素E处理的大鼠巨噬细胞中前列腺素E2生成的抑制作用。
Inhibition of PGE2 production in macrophages from vitamin E-treated rats.
作者信息
Sakamoto W, Fujie K, Nishihira J, Mino M, Morita I, Murota S
机构信息
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
出版信息
Prostaglandins Leukot Essent Fatty Acids. 1991 Oct;44(2):89-92. doi: 10.1016/0952-3278(91)90188-b.
Rat peritoneal macrophages from vitamin E-treated rats (5 mg per rat for 6 successive days) contained 403.3 +/- 90.7 ng alpha-tocopherol/10(6) cells, whereas control macrophages contained 1.2 +/- 0.4 ng. PGE2 production in the macrophages from vitamin E-treated rats was significantly suppressed when stimulated with PMA and calcium ionophore A23187. The mechanism of vitamin E inhibition of PGE2 production in macrophages was investigated. The release of (14C)-arachidonic acid from pre-labeled macrophages and the conversion of (14C)-arachidonic acid to PGE2 by the homogenate of the cells were remarkably reduced. These results strongly suggested that the inhibition of PGE2 production by vitamin E results from the inhibition of the activities of both phospholipase A2 and cyclooxygenase.
连续6天每天给大鼠喂食5毫克维生素E后，大鼠腹腔巨噬细胞每10⁶个细胞中含403.3±90.7纳克α-生育酚，而对照巨噬细胞仅含1.2±0.4纳克。用佛波酯（PMA）和钙离子载体A23187刺激时，来自维生素E处理大鼠的巨噬细胞中前列腺素E2（PGE2）的产生受到显著抑制。研究了维生素E抑制巨噬细胞中PGE2产生的机制。预先标记的巨噬细胞中（14C）-花生四烯酸的释放以及细胞匀浆将（14C）-花生四烯酸转化为PGE2的过程均显著减少。这些结果有力地表明，维生素E对PGE2产生的抑制作用源于对磷脂酶A2和环氧化酶活性的抑制。
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