Khamrin Pattara, Maneekarn Niwat, Peerakome Supatra, Tonusin Supin, Phan Tung Gia, Okitsu Shoko, Ushijima Hiroshi
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
J Med Virol. 2007 Jun;79(6):843-51. doi: 10.1002/jmv.20840.
In 2004, an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand detected two uncommon human rotavirus strains (CMH120/04 and CMH134/04) bearing AU-1-like G3P[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. The CMH120/04 and CMH134/04 rotavirus strains were characterized by molecular analyses of their VP6, VP7, VP8*, and NSP4 gene segments as well as the determination of RNA patterns by polyacrylamide gel electrophoresis (PAGE). Analysis of the VP8* gene revealed a high level of amino acid sequence identities with those of P[9] rotavirus reference strains, ranging from 94.9% to 98.3%. The highest identities were shared with the human rotavirus AU-1 strain at 97.8% and 98.3% for CMH120/04 and CMH134/04 strains, respectively. Analysis of the VP7 gene sequence revealed the highest identities with G3 human rotavirus strain KC814 at 96.6% and 96.2% for CMH120/04 and CMH134/04 strains, respectively. Based on the analyses of VP7 and VP8* genes, CMH120/04 and CMH134/04 belonged to G3P[9] genotypes. In addition, analyses of VP6 and NSP4 sequences revealed a VP6 subgroup (SG) I, with NSP4 genetic group C specificities. Moreover, both strains displayed a long RNA electrophoretic pattern. The finding of uncommon G3P[9] rotaviruses in pediatric patients provided additional evidence of the genetic/antigenic diversities of human group A rotaviruses in the Chiang Mai area of Thailand.
2004年，在泰国清迈开展的一项人类轮状病毒感染的流行病学调查中，在因急性肠胃炎住院的1岁儿童中检测到两种罕见的人类轮状病毒株（CMH120/04和CMH134/04），它们具有类似AU-1的G3P[9]基因型。通过对CMH120/04和CMH134/04轮状病毒株的VP6、VP7、VP8和NSP4基因片段进行分子分析，以及通过聚丙烯酰胺凝胶电泳（PAGE）测定RNA模式，对其进行了特征分析。VP8基因分析显示，与P[9]轮状病毒参考株的氨基酸序列同一性水平较高，范围为94.9%至98.3%。CMH120/04和CMH134/04株分别与人类轮状病毒AU-1株的同一性最高，分别为97.8%和98.3%。VP7基因序列分析显示，CMH120/04和CMH134/04株分别与G3人类轮状病毒株KC814的同一性最高，分别为96.6%和96.2%。基于VP7和VP8*基因分析，CMH120/04和CMH134/04属于G3P[9]基因型。此外，VP6和NSP4序列分析显示为VP6亚组（SG）I，具有NSP4基因组C特异性。而且，两种病毒株均呈现出长RNA电泳模式。在儿科患者中发现罕见的G3P[9]轮状病毒，为泰国清迈地区人类A组轮状病毒的遗传/抗原多样性提供了更多证据。
