Koç Aysel, Durkut Serap, Elçin A Eser, Tan Erdal, Elçin Y Murat
Ankara University, Faculty of Science and Biotechnology Institute, Tissue Engineering and Biomaterials Laboratory, Ankara 06100, Turkey.
Macromol Biosci. 2007 May 10;7(5):681-9. doi: 10.1002/mabi.200600265.
CMC and CMC-PVA were blended either with type I collagen, BSA or CS to obtain biocompatible membranes for evaluation as potential hepatocyte culture substrates. Pure and modified forms of CMC showed distinct surface, mechanical, and cell attachment properties. While the hydrophilicity decreased, the mechanical stability and the porosity of CMC membranes increased after blending. Serum proteins were adsorbed by all types of membranes. Among eight membranes tested, collagen-modified CMC was found to be a suitable membrane material for hepatocyte culture, in terms of mechanical and cell interaction properties.
将羧甲基纤维素（CMC）和羧甲基纤维素-聚乙烯醇（CMC-PVA）与I型胶原蛋白、牛血清白蛋白（BSA）或壳聚糖（CS）混合，以获得生物相容性膜，作为潜在的肝细胞培养底物进行评估。CMC的纯形式和改性形式表现出不同的表面、机械和细胞附着特性。混合后，CMC膜的亲水性降低，但其机械稳定性和孔隙率增加。所有类型的膜都能吸附血清蛋白。在测试的八种膜中，就机械和细胞相互作用特性而言，胶原蛋白改性的CMC被发现是一种适合肝细胞培养的膜材料。
