Daniluk T, Fiedoruk K, Sciepuk M, Zaremba M L, Rozkiewicz D, Cylwik-Rokicka D, Tokajuk G, Kedra B A, Anielska I, Stokowska W, Górska M, Kedra B R
Department of Microbiology, Medical University of Białystok, Poland.
Adv Med Sci. 2006;51 Suppl 1:86-90.
Determination of bacterial composition in the oral cavity of patients with removable dentures and with own dentition (without dentures).
Bacteriological investigations were performed in 55 patients from the department of internal medicine (32 diabetic patients) and 40 patients treated in surgical department (25 patients with malignancy). Palate mucosa and tongue dorsa swabs were collected from two groups of patients, and additionally swabs from mucosal part of denture surfaces in prosthetic patients. Cultures in oxygenic and microaerophilic (5% CO2) conditions were conducted on solid non-selective and selective media as well as media enriched with 5% sheep blood. Standard procedures of bacterial culture and identification were applied.
Among 95 of examined patients, 57 (60.0%) with removable dentures and 38 (40.0%) had their own dentition. As far as prosthetic patients were concerned, the rate of bacterial isolations from palate, tongue dorsa and denture plaque swabs were generally comparable (p > 0.05); in number and species compositions. Statistically significant differences were observed in the bacterial composition of denture plaques, palate and tongue dorsa in patients with and without abdominal cancers. Patients without cancer did not reveal staphylococci and enteric bacteria in the samples from a various sites of their oral cavities. These bacteria were most common in cancer patients. Similar (in number and species) composition of bacteria occurred in palate and tongue swabs in patients without dentures (p > 0.05). The incidence rate of aerobic bacteria in denture plaques and palatal mucosa of patients with (37/57; 64.9%) and without (20/57; 35.1%) denture associated stomatitis were comparable (except for Neisseria spp.).
确定佩戴可摘义齿患者与天然牙列（无义齿）患者口腔中的细菌组成。
对内科的55例患者（32例糖尿病患者）和外科治疗的40例患者（25例恶性肿瘤患者）进行了细菌学调查。从两组患者中采集腭黏膜和舌背拭子，此外还从修复患者义齿表面的黏膜部分采集拭子。在固体非选择性和选择性培养基以及添加5%羊血的培养基上，在有氧和微需氧（5%二氧化碳）条件下进行培养。采用标准的细菌培养和鉴定程序。
在95例受检患者中，57例（60.0%）佩戴可摘义齿，38例（40.0%）为天然牙列。就修复患者而言，从腭部、舌背和义齿菌斑拭子中分离出细菌的比率在数量和种类组成上总体相当（p>0.05）。在有和没有腹部癌症的患者中，义齿菌斑、腭部和舌背的细菌组成存在统计学显著差异。无癌症患者在其口腔不同部位的样本中未发现葡萄球菌和肠道细菌。这些细菌在癌症患者中最为常见。无义齿患者的腭部和舌部拭子中细菌组成相似（在数量和种类上）（p>0.05）。有义齿相关口腔炎患者（37/57；64.9%）和无义齿相关口腔炎患者（20/57；35.1%）的义齿菌斑和腭黏膜中需氧菌的分离率相当（除奈瑟菌属外）。
1）一般来说，佩戴和未佩戴可摘义齿患者从硬腭和舌背分离出的细菌种类组成没有统计学显著差异。2）与无癌症患者相比，癌症患者的义齿菌斑、腭部和舌背更常分离出葡萄球菌属和革兰氏阴性肠道杆菌（p<0.05）。3）与非糖尿病患者相比，糖尿病患者的义齿菌斑中更频繁地分离出葡萄球菌属（p<0.05）。4）有和无义齿相关口腔炎患者的义齿菌斑和腭黏膜中需氧菌的分离频率（%）没有显著差异。
