成人根龋损害中的微生物

Zaremba M L, Stokowska W, Klimiuk A, Daniluk T, Rozkiewicz D, Cylwik-Rokicka D, Waszkiel D, Tokajuk G, Kierklo A, Abdelrazek S

Department of Microbiology, Medical University of Białystok, Poland.

Adv Med Sci. 2006;51 Suppl 1:237-40.

PURPOSE

Root caries is emerging as a significant problem in the middle aged and elderly subjects because of the improving general health conditions, and medical and technological advances. The purpose of this investigation was to assess the prevalence of aerobic and anaerobic bacteria as well as yeasts of Candida genus in root carious lesions in middle-aged and older adults.

MATERIAL AND METHODS

Specimens of root carious lesions were collected from 78 adults for bacteriological and mycological studies. Standard procedures of culture, isolation, and identification of aerobic and anaerobic bacteria, and fungi were used in the study.

RESULTS

The analysis of results was performed independently in two age groups of adults, i.e. 52 subjects aged 35-44 years (middle age) and 26-aged 55-72 years (older age). There were 120 bacterial strains isolated from root carious lesions in middle-aged subjects, 63 (52.5%) strains belonged to 5 genera of aerobic bacteria and 57 (47.5%)--to 7 genera of anaerobic bacteria (p > 0.05). While in the second group, 85 strains were isolated, 54 (63.5%)--6 genera of aerobic bacteria and 31 (36.5%)--4 genera of anaerobic bacteria (p = 0.0004). There were no differences between the isolation rate of a various species in both examined groups, except for Streptococcus spp., S. oralis, Micrococcus spp., Neisseria spp. and Veillonella spp., which statistically significantly most frequent occurred in elderly (p < 0.05). The yeasts of Candida genus of 4 species (C. albicans, C. lusitaniae, C. pelliculosa, and C. pulcherrima) were isolated from middle-aged subjects (32.7%) with the comparable rate to older adults (30.8%; only C. albicans) (p > 0.05). Among all isolated microorganisms, Candida spp., were comprised about 10% in both examined groups (p > 0.05).

CONCLUSIONS

Aerobic Gram-positive cocci (Staphylococcus spp. and Streptococcus spp.) as well as anaerobic ones (Peptostreptococcus spp.), and Candida albicans were occurred most frequently in root carious lesions in middle-aged and older adults.

目的

由于总体健康状况的改善以及医学和技术的进步，根龋在中老年人群中已成为一个重要问题。本研究的目的是评估中老年成年人根龋病变中需氧菌、厌氧菌以及念珠菌属酵母菌的患病率。

材料与方法

从78名成年人中收集根龋病变标本进行细菌学和真菌学研究。研究采用需氧菌、厌氧菌和真菌培养、分离及鉴定的标准程序。

结果

对两个年龄组的成年人独立进行结果分析，即52名年龄在35 - 44岁的成年人（中年组）和26名年龄在55 - 72岁的成年人（老年组）。中年组根龋病变中分离出120株细菌菌株，其中63株（52.5%）属于5个需氧菌属，57株（47.5%）属于7个厌氧菌属（p > 0.05）。而在老年组中，分离出85株菌株，其中54株（63.5%）属于6个需氧菌属，31株（36.5%）属于4个厌氧菌属（p = 0.0004）。除口腔链球菌、微球菌属、奈瑟菌属和韦荣球菌属外，两个检查组中各菌种的分离率无差异，这些菌在老年人中出现的频率在统计学上显著更高（p < 0.05）。从中年组中分离出4种念珠菌属酵母菌（白色念珠菌、葡萄牙念珠菌、膜状念珠菌和美艳念珠菌）（32.7%），与老年成年人的分离率相当（30.8%；仅白色念珠菌）（p > 0.05）。在所有分离出的微生物中，念珠菌属在两个检查组中均约占10%（p > 0.05）。