Li Aiduan, Antizar-Ladislao Blanca, Khraisheh Majeda
Department of Civil and Environmental Engineering, University College London, London, UK.
Bioprocess Biosyst Eng. 2007 May;30(3):189-96. doi: 10.1007/s00449-007-0114-3. Epub 2007 Feb 15.
Selected biodegradable municipal solid waste fractions were subjected to fifteen different pre-hydrolysis treatments to obtain the highest glucose yield for bio-ethanol production. Pre-hydrolysis treatments consisted of dilute acid (H(2)SO(4), HNO(3) or HCl, 1 and 4%, 180 min, 60 degrees C), steam treatment (121 and 134 degrees C, 15 min), microwave treatment (700 W, 2 min) or a combination of two of them. Enzymatic hydrolysis was carried out with Trichoderma reesei and Trichoderma viride (10 and 60 FPU g(-1) substrate). Glucose yields were compared using a factorial experimental design. The highest glucose yield (72.80%) was obtained with a pre-hydrolysis treatment consisting of H(2)SO(4) at 1% concentration, followed by steam treatment at 121 degrees C, and enzymatic hydrolysis with Trichoderma viride at 60 FPU g(-1) substrate. The contribution of enzyme loading and acid concentration was significantly higher (49.39 and 47.70%, respectively), than the contribution of temperature during steam treatment (0.13%) to the glucose yield.
选取了可生物降解的城市固体废弃物部分，对其进行15种不同的预水解处理，以获得用于生物乙醇生产的最高葡萄糖产率。预水解处理包括稀酸（硫酸、硝酸或盐酸，1%和4%，180分钟，60℃）、蒸汽处理（121℃和134℃，15分钟）、微波处理（700瓦，2分钟）或其中两种处理的组合。使用里氏木霉和绿色木霉（10和60 FPU g-1底物）进行酶水解。采用析因实验设计比较葡萄糖产率。通过由1%浓度的硫酸进行预水解处理、随后在121℃进行蒸汽处理以及使用60 FPU g-1底物的绿色木霉进行酶水解，获得了最高葡萄糖产率（72.80%）。酶负载量和酸浓度对葡萄糖产率的贡献显著更高（分别为49.39%和47.70%），高于蒸汽处理过程中温度对葡萄糖产率的贡献（0.13%）。
