Madej Paweł, Plewka Andrzej, Madej Janusz A, Plewka Danuta, Mroczka Wojciech, Wilk Krzysztof, Dobrosz Zuzanna
Department and Clinic of Gynaecological Endocrynology, Medical University of Silesia, ul. Medyków 14, 40-752, Katowice, Poland.
Inflammation. 2007 Aug;30(3-4):69-86. doi: 10.1007/s10753-007-9023-5. Epub 2007 Apr 26.
Mitochondria and lysosomes were evaluated by assessment of changes in activity of selected enzymes: lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), adenosinetriphosphatase (ATPase), acid phosphatase (AcPase) and beta-glucuronidase (BG) in rats under profound hypoxia induced by endotoxemic shock. The study was conducted on adult male Wistar rats. The animals formed the following four groups of 15 rats each: control animals (C);-rats receiving intraperitonally O(2)/O(3) (CO), rats receiving of Escherichia coli toxin (LPS) (CL); rats receiving LPS plus oxygen-ozone mixture (OL). Histoenzymatic examinations of liver, kidney, lungs, and heart muscle were performed. Lipopolysaccharide suppressed activities of all the enzymes except for LDH, the activity of which as high as a fourfold increase. The results demonstrated potent, stabilizing and regenerative effects of ozone therapy on body enzymatic processes in course of induced endotoxemic shock in rats, which might prove to be of clinical significance.
通过评估内毒素血症休克诱导的深度缺氧大鼠体内选定酶的活性变化，来评估线粒体和溶酶体：乳酸脱氢酶（LDH）、琥珀酸脱氢酶（SDH）、三磷酸腺苷酶（ATPase）、酸性磷酸酶（AcPase）和β - 葡萄糖醛酸酶（BG）。该研究以成年雄性Wistar大鼠进行。动物分为以下四组，每组15只大鼠：对照组动物（C）；腹腔注射O₂/O₃的大鼠（CO），接受大肠杆菌毒素（LPS）的大鼠（CL）；接受LPS加氧 - 臭氧混合物的大鼠（OL）。对肝脏、肾脏、肺和心肌进行组织酶学检查。脂多糖抑制了除LDH之外所有酶的活性，而LDH的活性增加高达四倍。结果表明，臭氧疗法对大鼠诱导性内毒素血症休克过程中的身体酶促过程具有强大的稳定和再生作用，这可能具有临床意义。
