Degoricija Lovorka, Johnson C Starck, Wathier Michel, Kim Terry, Grinstaff Mark W
Departments of Chemistry, Boston University, Boston, Massachusetts, USA.
Invest Ophthalmol Vis Sci. 2007 May;48(5):2037-42. doi: 10.1167/iovs.06-0957.
Biodendrimer-based hydrogel adhesives were derived from biocompatible building blocks and poly(ethylene glycol) of 3,400, 10,000 and 20,000 g/mole. The leaking pressures were determined for these adhesives when used to seal 4.1-mm central lacerations and penetrating keratoplasties (PKPs) in enucleated porcine eyes.
Three biodendrimers, ([G1]-PGLSA-MA)(2)-PEG(3,400), ([G1]-PGLSA-MA)(2)-PEG(10,000), and ([G1]-PGLSA-MA)(2)-PEG(20,000), at a range of weight percents were each photo cross-linked in the presence of a photo-initiator to form a hydrated network. These biodendrimer-based adhesives were applied directly to a 4.1-mm linear central laceration. In a PKP, the corneal button was initially secured with 8 or 16 sutures and then sealed with the adhesive.
For the 4.1-mm central lacerations, the ([G1]-PGLSA-MA)(2)-PEG(3,400) at 20% and 40% wt/vol, the ([G1]-PGLSA-MA)(2)-PEG(10,000) at 10 and 20% wt/vol, and the ([G1]-PGLSA-MA)(2)-PEG(20,000) at 20% wt/vol held to leaking pressures above 200 mm Hg. In the autograft with 16 sutures, the 20% wt/vol of the ([G1]-PGLSA-MA)(2)-PEG(3,400), ([G1]-PGLSA-MA)(2)-PEG(10,000), and ([G1]-PGLSA-MA)(2)-PEG(20,000) held to a pressure at or above 100 mm Hg. In the autograft with eight sutures, the ([G1]-PGLSA-MA)(2)-PEG(10,000) and ([G1]-PGLSA-MA)(2)-PEG(20,000) formulations at 20% wt/vol held to leaking pressures of 85 +/- 22 and 80 +/- 30 mm Hg, respectively.
The 10% wt/vol ([G1]-PGLSA-MA)(2)-PEG(10,000) formulation withheld leaking pressures above 200 mm Hg when used to secure a 4.1 mm central laceration. The 20% wt/vol ([G1]-PGLSA-MA)(2)-PEG(10,000) and ([G1]-PGLSA-MA)(2)-PEG(20,000) formulations, with 8 or 16 sutures, secured the PKP well above normal IOP. Biodendrimer-based adhesives are of potential use for repairing corneal wounds.
基于生物树枝状大分子的水凝胶粘合剂由生物相容性构建块和分子量分别为3400、10000和20000 g/mol的聚乙二醇衍生而来。测定了这些粘合剂用于封闭摘除的猪眼中4.1毫米中央撕裂伤和穿透性角膜移植术（PKP）时的渗漏压力。
三种生物树枝状大分子，([G1]-PGLSA-MA)(2)-PEG(3400)、([G1]-PGLSA-MA)(2)-PEG(10000)和([G1]-PGLSA-MA)(2)-PEG(20000)，在一系列重量百分比下，分别在光引发剂存在下进行光交联以形成水合网络。这些基于生物树枝状大分子的粘合剂直接应用于4.1毫米线性中央撕裂伤。在PKP中，角膜片最初用8或16根缝线固定，然后用粘合剂封闭。
对于4.1毫米中央撕裂伤，20%和40%重量/体积的([G1]-PGLSA-MA)(2)-PEG(3400)、10%和20%重量/体积的([G1]-PGLSA-MA)(2)-PEG(10000)以及20%重量/体积的([G1]-PGLSA-MA)(2)-PEG(20000)的渗漏压力保持在200毫米汞柱以上。在有16根缝线的自体移植中，20%重量/体积的([G1]-PGLSA-MA)(2)-PEG(3400)、([G1]-PGLSA-MA)(2)-PEG(10000)和([G1]-PGLSA-MA)(2)-PEG(20000)的压力保持在100毫米汞柱或以上。在有8根缝线的自体移植中，20%重量/体积的([G1]-PGLSA-MA)(2)-PEG(10000)和([G1]-PGLSA-MA)(2)-PEG(20000)配方的渗漏压力分别为85±22和80±30毫米汞柱。
10%重量/体积的([G1]-PGLSA-MA)(2)-PEG(10000)配方用于固定4.1毫米中央撕裂伤时，渗漏压力保持在200毫米汞柱以上。20%重量/体积的([G1]-PGLSA-MA)(2)-PEG(10000)和([G1]-PGLSA-MA)(2)-PEG(20000)配方，在有8或16根缝线时，能将PKP固定在高于正常眼压的水平。基于生物树枝状大分子的粘合剂在修复角膜伤口方面具有潜在用途。
